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Module Specifications..

Current Academic Year 2023 - 2024

Please note that this information is subject to change.

Module Title Gene Cloning and Gene Expression
Module Code BE301
School School of Biotechnology
Module Co-ordinatorSemester 1: Anne Parle-McDermott
Semester 2: Anne Parle-McDermott
Autumn: Anne Parle-McDermott
Module TeachersPaul Cahill
Paula Meleady
Anne Parle-McDermott
Naomi Walsh
Denise Harold
Linda Holland
Janosch Heller
NFQ level 8 Credit Rating 5
Pre-requisite None
Co-requisite None
Compatibles None
Incompatibles None
Repeat examination

To develop a good understanding of the biology of bacteriophages and plasmids. To teach the basis of techniques used in the manipulation of DNA/RNA and to provide a theoretical background that would enable students to learn the skills involved in nucleic acid manipulation. To develop an appreciation and understanding of the complexity of gene expression and its control, with an emphasis on eukaryotic systems. To give an overview of the content of Eukaryotic genomes and their functionality.

Learning Outcomes

1. Design and understand recombinant DNA cloning strategies and other relevant molecular biological methodologies useful in the investigation of nucleic acids.
2. Select the appropriate vector system for application in genetic analysis
3. Analyse genome sequences to gain an understanding of gene organisation and function of the encoded information- a focus on the gene itself as a functional unit
4. Design an experimental approach to monitor the expression of genes using RNA analysis
5. Gain knowledge and understanding of the complexity of Eukaryotic Gene Expression control.

Workload Full-time hours per semester
Type Hours Description
Tutorial12No Description
Lecture12No Description
Independent Study101Reading recommended text books and working through recorded lectures.
Total Workload: 125

All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml

Indicative Content and Learning Activities

Recombinant DNA technology

- restriction enzymes, joining DNA molecules. DNA amplification, the polymerase chain reaction. Vectors for gene cloningPlasmids, introduction to plasmids

- structure, high and low copy number, different forms, plasmid encoded phenotypes.Replication of plasmids

DNA sequencing methodologies.

Assessment Breakdown
Continuous Assessment100% Examination Weight0%
Course Work Breakdown
TypeDescription% of totalAssessment Date
Participation10% of overall mark for participation over the 12 weeks.10%n/a
Loop Quiz1 Loop quiz worth 10% covering the first half of BE30110%Week 6
Essay1 Essays worth 35% covering the first part of BE30135%n/a
Loop Quiz1 Loop quiz worth 10% covering the second half of BE30110%Week 12
Essay1 Essay worth 35% covering the second part of BE30135%n/a
Reassessment Requirement Type
Resit arrangements are explained by the following categories;
1 = A resit is available for all components of the module
2 = No resit is available for 100% continuous assessment module
3 = No resit is available for the continuous assessment component
This module is category 1
Indicative Reading List

  • Jeremy W. Dale, Simon Park: 0, Molecular genetics of bacteria, 0470850841
  • Richard J. Reece: 0, Analysis of genes and genomes, 0470843799
  • T. A. Brown: 0, Genetics, 0412447304
  • Lewin: 0, GENES XII,
  • Strachan and Read: 0, Human Molecular Genetics,
Other Resources

Programme or List of Programmes
AFUAge Friendly University Programme
ASBSc in Analytical Science
BPBSc in Bioprocessing
BSSAStudy Abroad (DCU Business School)
BSSAOStudy Abroad (DCU Business School)
BTBSc in Biotechnology
GCBBSc in Genetics & Cell Biology
HMSAStudy Abroad (Humanities & Soc Science)
HMSAOStudy Abroad (Humanities & Soc Science)
IESAStudy Abroad (Institute of Education)
IESAOStudy Abroad (Institute of Education)
SHSAStudy Abroad (Science & Health)
SHSAOStudy Abroad (Science & Health)
Date of Last Revision23-SEP-05

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