Module Specifications..
Current Academic Year 2023 - 2024
Please note that this information is subject to change.
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Repeat examination Array |
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Description The purpose of this module is to provide students with the skills and understanding necessary for handling and quantitation of micro-organisms in a laboratory environment. To this end, you will work through Standard Operating Procedures commonly used in food and water microbiology and use procedures for surfaces to quantify microbial contamination. Additionally, you will use standard biochemical techniques to purify a protein from egg. This is a continuously assessed laboratory module and attendance is compulsory and will be monitored. | |||||||||||||||||||||||||||||||||||||||||||||
Learning Outcomes 1. Analyse food and water samples for microbes and use these findings to determine the quality of food and water samples. 2. Culture microbes in batch and continuous culture, graphically analyse the results and use these to calculate microbial growth parameters. 3. Carry out a sequential 'multistep' protein purification scheme including ion-exchange and gel filtration chromatography and calculate yield and purification. 4. Write up reports, to be viewed by an experimental scientist and by the general public, detailing their experimental findings and the conclusions that may be drawn from them. | |||||||||||||||||||||||||||||||||||||||||||||
All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml |
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Indicative Content and Learning Activities
Assay for lyzozyme activity and proteinPreparation of crude lyzozyme from egg-whiteSalt precipitation of lyzozyme by gel-filtration chromatography on sephadex G-100Purification of 'partially-purified' lyzozyme by cation-exchange chromatographyConcentration of lyzozyme by reverse dialysis using solid polyethylene glycolAnalysis of lyzozyme fractions by native and SDS Polyacrylamide electrophoresisAnalysis of lyzozyme fractions and standards by Isoelectric focusingComputer learning system for teaching the use of HPLC and its applicationsTechniques to prepare and analyse samples for the HPLC analysis of drugsDetermination of molar growth yield in batch and continuous microbial fermentationFood microbiology - analysis of samples by pour plates and spread platesWater microbiology - analysis of samples by MPN and membrane filtration | |||||||||||||||||||||||||||||||||||||||||||||
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Indicative Reading List
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Other Resources None | |||||||||||||||||||||||||||||||||||||||||||||
Programme or List of Programmes
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Date of Last Revision | 01-OCT-08 | ||||||||||||||||||||||||||||||||||||||||||||
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