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Module Specifications..

Current Academic Year 2023 - 2024

Please note that this information is subject to change.

Module Title Cell Biol.,Recombinant DNA Cloning & Bioinf.
Module Code BE357
School School of Biotechnology
Module Co-ordinatorSemester 1: Ronan O'Toole
Semester 2: Ronan O'Toole
Autumn: Ronan O'Toole
Module TeachersPaul Cahill
Anne Parle-McDermott
Jiafu Wang
Denise Harold
Emma Finlay
Linda Holland
Ronan O'Toole
NFQ level 8 Credit Rating 5
Pre-requisite None
Co-requisite None
Compatibles None
Incompatibles None
Repeat the module

This module will provide a detailed understanding of the methods used in: (i) mammalian cell biology including basic cell culture techniques, cell counting, seeding growth and cell viability studies in addition to training in techniques for assessment of cell differentiation (phenotype) in situ (immunocytochemistry), cell signalling and protein expression (Western blotting) and cell secretion of proteins (ELISA-enzyme-linked immunosorbent assay). (ii) The basic practical techniques of Polymerase Chain Reaction, recombinant DNA technology and to develop their understanding of strategies used in the construction, confirmation and expression of recombinant molecules in bacterial cells. Bioinformatics will include sequence retrieval and analysis of protein coding sequences. Attendance at practical sessions is mandatory and students are expected to engage in tutorial sessions and complete assignments.

Learning Outcomes

1. Perform routine cell culture using adherent mammalian cells to learn basic cell counting, cell adherence growth and viability assays.
2. Measure the phenotype and differentiation state of a cell using fluorescent immunocytochemistry in situ.
3. Measure the concentration of an intracellular protein in a cell lysate.
4. Perform SDS-PAGE gel electrophoresis and Western blot analysis to prove for the presence of specific intracellular proteins.
5. Perform an Enzyme Linked Immunosorbent Assay (ELISA) of secreted proteins from cells.
6. Perform basic recombinant DNA cloning techniques.
7. Use specific genomic databases and bioinformatics software packages.
8. Design an experiment to generate a recombinant DNA clone of a gene of interest using PCR.
9. Interpret restriction maps of recombinant DNA molecules.

Workload Full-time hours per semester
Type Hours Description
Laboratory48Series of practicals related to cell biology methods and recombinant DNA cloning.
Assignment Completion15Bioinformatics assignment
Directed learning10Report writing
Independent Study52Reading of specified materials and independent learning time.
Total Workload: 125

All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml

Indicative Content and Learning Activities

Mammalian cell culture and assessment of growth and viability.

The techniques of fluorescent in situ immunocytochemistry, protein concentration determination, Western blotting & ELISA

Recombinant DNA cloning in E.coli
Recombinant DNA cloning in E.coli and includes the following techniques: PCR, gel electrophoresis, Gateway cloning technology, bacterial transformation and selection, restriction enzyme mapping.

Access and analyse a specific protein coding DNA sequence of interest.

Assessment Breakdown
Continuous Assessment100% Examination Weight0%
Course Work Breakdown
TypeDescription% of totalAssessment Date
Practical/skills evaluationAbility to perform experimental protocols in a standard laboratory environment.20%n/a
Report(s)Lab report to be submitted where individual group and class data is presented and discussed.65%n/a
AssignmentAn assignment to access appropriate databases and software tools to design a recombinant DNA cloning experiment.15%n/a
Reassessment Requirement Type
Resit arrangements are explained by the following categories;
1 = A resit is available for all components of the module
2 = No resit is available for 100% continuous assessment module
3 = No resit is available for the continuous assessment component
This module is category 2
Indicative Reading List

    Other Resources

    40470, 0, Lab manuals and associated articles and resources available on Loop.,
    Programme or List of Programmes
    GCBBSc in Genetics & Cell Biology

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