Module Specifications..
Current Academic Year 2023 - 2024
Please note that this information is subject to change.
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Repeat the module Array |
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Description To introduce students to applications of Web based bioinformatic analyses To train students in the basic practical techniques of recombinant DNA technology and to develop their understanding of strategies used in the construction, mapping and expression and purification of recombinant molecules. | |||||||||||||||||||||||||||||||||||||||||
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Learning Outcomes 1. Apply computational skills to analyse DNA sequences using bioinformatics tools 2. Construct recombinant DNA molecules using the techniques of gene cloning 3. Analyse DNA structure and gene expression using restriction digestion and phenotypic detection 4. Purify a recombinant protein using affinity chromatography 5. Record laboratory research results through the maintenance of a laboratory notebook, accurately reporting experimental results and conclusions. | |||||||||||||||||||||||||||||||||||||||||
All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml |
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Indicative Content and Learning Activities
OverviewOverview of bioinformatics and nucleotide and protein databases. Derivation and format of nucleotide and protein sequences e.g. FASTA. Using web resources e.g. NCBI to access and retrieve sequences. Comparing DNA and protein sequences with appropriate databases using BLAST. Displaying similarities using alignments and multiple alignmentsIdentification of coding regions and consensus sequences. Translating nucleotide sequences and searching for open reading frames. Analysis of nucleotide sequences for restriction enzyme sites. Primer design for the PCR. Application of PCR for the amplification of target genes. Analysis of DNA by agarose gel electrophoresis. DNA restriction and restriction mappingCloning in plasmid vectorsLigation. Transformation and electroporation. Extraction of plasmid DNA from transformants and its analysis by restriction and gel electrophoresis. Site directed mutagenesis. Purification of a recombinant protein using a one step affinity chromatography. | |||||||||||||||||||||||||||||||||||||||||
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Indicative Reading List | |||||||||||||||||||||||||||||||||||||||||
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Other Resources None | |||||||||||||||||||||||||||||||||||||||||
Programme or List of Programmes
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| Date of Last Revision | 15-MAY-08 | ||||||||||||||||||||||||||||||||||||||||
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