Module: Bioanalytical Practicals II

Module Specifications..

Current Academic Year 2023 - 2024

Please note that this information is subject to change.

Module Title	Bioanalytical Practicals II
Module Code	BE452
School	School of Biotechnology
Module Co-ordinator	Semester 1: Christine Loscher Semester 2: Christine Loscher Autumn: Christine Loscher
Module Teachers	Paul Cahill Tom Zurawski Christine Loscher Denise Harold
NFQ level	8	Credit Rating	5
Pre-requisite	None
Co-requisite	None
Compatibles	None
Incompatibles	None

None
Array

Description

This module builds on an understanding of concepts in molecular biology and immunology. It aims to teach a range of methods in these subject areas by providing training in selected relevant techniques.

Learning Outcomes

1. isolate, quantitate and analyse nucleic acid preparations from various sources
2. Qualitatively analyse a Single Nucleotide Polymorphism-containing DNA sequence using methods including Polymerase Chain Reaction, hybridisation probes, restriction endonuclease analysis and electrophoresis
3. isolate and characterise polyclonal antibodies and conjugate antibodies with enzyme labels

*Type*	*Hours*	*Description*
Workload	Full-time hours per semester
Laboratory	48	Hybrid format - pre-recorded material, laboratory work and online discussion forums
Independent Study	27	Data interpretation and report writing
Directed learning	50	Preparation for assessment
Total Workload: 125

All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml

Indicative Content and Learning Activities

Preparation and analysis of mammalian DNA

Single Nucleotide Polymorphism characterisation by PCR/hybridisation probes

Single Nucleotide Polymorphism characterisation by PCR/RFLP

Immunoglobulins will be isolated from animal serum, chemically conjugated to enzymes and used in enzyme linked immunosorbant assays

Assessment Breakdown
Continuous Assessment	100%	Examination Weight	0%

Type	Description	% of total	Assessment Date
Course Work Breakdown
In Class Test	Data interpretation and experimental design	50%	n/a
Laboratory Portfolio	Experimental write-up	50%	n/a

Reassessment Requirement Type
Resit arrangements are explained by the following categories; 1 = A resit is available for all components of the module 2 = No resit is available for 100% continuous assessment module 3 = No resit is available for the continuous assessment component
This module is category 2

Indicative Reading List

Other Resources

None

Programme or List of Programmes

AS	BSc in Analytical Science

Date of Last Revision

26-MAY-08

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