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Module Specifications..

Current Academic Year 2023 - 2024

Please note that this information is subject to change.

Module Title Advanced Bioanalysis Laboratory
Module Code BE454
School School of Biotechnology
Module Co-ordinatorSemester 1: Paula Meleady
Semester 2: Paula Meleady
Autumn: Paula Meleady
Module TeachersPaul Cahill
Paula Meleady
Phil Cummins
Denise Harold
Linda Holland
NFQ level 8 Credit Rating 5
Pre-requisite None
Co-requisite None
Compatibles None
Incompatibles None
Coursework Only

The purpose of this module is to prepare the students in advanced laboratory techniques that are relevant to the Biopharma industry. The students will develop a good understanding of the theory and practice of analytical instrumentation. They will also be able to: 1. Measure the concentration and purity of a drug using HPLC 2. Perform basic techniques in animal cell culture 3. Measure the impact of a chemotherapeutic agent on cancer cell growth 4. Use a range of immunological techniques to examine immune suppressive properties of a novel immune therapeutic agent 5. Evaluate and critique research data from experiments in cell and molecular biology

Learning Outcomes

1. Theory and practice of analytical instrumentation: Understand the basic chromatographic principles of bioanalysis using high performance liquid chromatography (HPLC, UHPLC) and explain the critical functional components of a HPLC system. Carry out a HPLC analysis to determine the concentration/purity of an analyte (e.g. a drug or metabolite) in a biological sample or other preparation. Understand how HPLC parameters (e.g. mobile phase composition, flow rate, sample preparation) can be practically optimized to improve sample separation and quantitation.
2. Basic techniques in animal cell culture: Work aseptically with a human colon cancer cell line. Perform a direct cell count and distinguish between viable and dead cells. Implement the technical aspects that are important for animal cell culture.
3. Chemotherapeutic agent on cancer cell growth: Set up 96-well microtitre plates to measure the impact of a chemotherapeutic agent on cell viability, cytotoxicity and cell proliferation.
4. Immune suppressive properties of a novel drug candidate: Set up Enzyme-linked immunosorbent assay to measure cytokine release. Digital interactive session on Flow Cytometry
5. Write concise and informative laboratory reports.

Workload Full-time hours per semester
Type Hours Description
Total Workload: 0

All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml

Indicative Content and Learning Activities

Lab 1:Basic Techniques in Animal Cell culture
Establish the growth curve of SW480 cells. Lab 2: Perform MTS and Cyquant Cell proliferation assays to establish effects of drug candidate on cell growth. Lab 4: Workshop on establishing a high throughput automated cancer cell proliferation panel for optimizing oncology drug discovery. Tutorial on data presentation and interpretation

Lab 2: Theory and practice of analytical Instrumentation.
HPLC theory, analysis and practice;

Workshop I: Virtual ELISA
Virtual Enzyme-linked immunosorbent assay. Workshop on data interpretation and presentation

Workshop II: Virtual Flow cytometry
Virtual demonstration of Flow Cytometer. Workshop on data interpretation and critique

Assessment Breakdown
Continuous Assessment100% Examination Weight0%
Course Work Breakdown
TypeDescription% of totalAssessment Date
Report(s)Basic Techniques in Cell Culture25%Week 24
Report(s)HPLC report25%Week 27
Completion of online activityELISA and Flow Cytometry online assignments50%Sem 2 End
Reassessment Requirement Type
Resit arrangements are explained by the following categories;
1 = A resit is available for all components of the module
2 = No resit is available for 100% continuous assessment module
3 = No resit is available for the continuous assessment component
This module is category 2
Indicative Reading List

  • John R. W. Masters: 2000, Animal cell culture, 3rd ed, Oxford University Press, Oxford, 0199637962
  • Freshney: 2010, Culture of animal cells: a manual of basic technique and specialized applications, 6th, John Wiley & Sons, Hoboken, N.J.: Wiley-Blackwell, 0470528125,
Other Resources

Programme or List of Programmes
BTBSc in Biotechnology
GCBBSc in Genetics & Cell Biology

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