Module: Introduction to Proteomics & Mass Spectrometry

Module Specifications.

Current Academic Year 2024 - 2025

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Date posted: September 2024

Module Title	Introduction to Proteomics & Mass Spectrometry
Module Code	BE532 (ITS) / BTE1027 (Banner)
Faculty	Science & Health	School	Biotechnology
Module Co-ordinator	Paula Meleady
Module Teachers	Finbarr O'Sullivan
NFQ level	9	Credit Rating	2.5
Pre-requisite	Not Available
Co-requisite	Not Available
Compatibles	Not Available
Incompatibles	Not Available

None

Description

The purpose of this module is to introduce participants to the technology currently available to analyse global patterns of gene expression at the protein level. The module will addresss applications of this technology to reveal gene regulation events involved in disease progression as well as potential targets for drug discovery and diagnostics. The module will cover the following aspects of proteomic technologies and research; • Sample preparation from various biological materials (e.g. cell lines, tissue, serum, plasma, etc.) • 2D Gel Electrophoresis, including 2D Difference Gel Electrophoresis (2D DIGE) • Mass Spectrometry (MS) for protein identification and characteristion • Quantitative LC-MS proteomic technologies o SILAC o iTRAQ o Label-free • Introduction to bioinformatics tools for proteomics

Learning Outcomes

1. Select the most appropriate method of sample preparation for various biological materials.
2. Discuss the advantages, limitations and challenges associated with analysis of the proteome by different techniques.
3. Demonstrate knowledge of the differences between the various mass spectrometry techniques for protein identification and characterization.
4. Have an ability to analyze and characterize a protein of interest using various protein bioinformatic software tools.

*Type*	*Hours*	*Description*
Workload	Full-time hours per semester
Lecture	12	No Description
Independent Study	3	Reading
Directed learning	1	No Description
Total Workload: 16

All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml

Indicative Content and Learning Activities

None

Assessment Breakdown
Continuous Assessment	0%	Examination Weight	0%

Type	Description	% of total	Assessment Date
Course Work Breakdown
Short Answer Questions	n/a	100%

Reassessment Requirement Type
Resit arrangements are explained by the following categories: Resit category 1: A resit is available for both* components of the module. Resit category 2: No resit is available for a 100% continuous assessment module. Resit category 3: No resit is available for the continuous assessment component where there is a continuous assessment and examination element.
* ‘Both’ is used in the context of the module having a Continuous Assessment/Examination split; where the module is 100% continuous assessment, there will also be a resit of the assessment
This module is category -

Indicative Reading List

Other Resources

None