DCU Home | Our Courses | Loop | Registry | Library | Search DCU

Module Specifications..

Current Academic Year 2023 - 2024

Please note that this information is subject to change.

Module Title Introduction to Proteomics &Mass Spectrometry
Module Code BE532
School School of Biotechnology
Module Co-ordinatorSemester 1: Paula Meleady
Semester 2: Finbarr O'Sullivan
Autumn: Finbarr O'Sullivan
Module TeachersPaula Meleady
Finbarr O'Sullivan
NFQ level 9 Credit Rating 2.5
Pre-requisite None
Co-requisite None
Compatibles None
Incompatibles None

The purpose of this module is to introduce participants to the technology currently available to analyse global patterns of gene expression at the protein level. The module will addresss applications of this technology to reveal gene regulation events involved in disease progression as well as potential targets for drug discovery and diagnostics. The module will cover the following aspects of proteomic technologies and research; • Sample preparation from various biological materials (e.g. cell lines, tissue, serum, plasma, etc.) • 2D Gel Electrophoresis, including 2D Difference Gel Electrophoresis (2D DIGE) • Mass Spectrometry (MS) for protein identification and characteristion • Quantitative LC-MS proteomic technologies o SILAC o iTRAQ o Label-free • Introduction to bioinformatics tools for proteomics

Learning Outcomes

1. Select the most appropriate method of sample preparation for various biological materials.
2. Discuss the advantages, limitations and challenges associated with analysis of the proteome by different techniques.
3. Demonstrate knowledge of the differences between the various mass spectrometry techniques for protein identification and characterization.
4. Have an ability to analyze and characterize a protein of interest using various protein bioinformatic software tools.

Workload Full-time hours per semester
Type Hours Description
Lecture12No Description
Independent Study3Reading
Directed learning1No Description
Total Workload: 16

All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml

Indicative Content and Learning Activities

Assessment Breakdown
Continuous Assessment100% Examination Weight0%
Course Work Breakdown
TypeDescription% of totalAssessment Date
Short Answer Questionsn/a100%
Reassessment Requirement Type
Resit arrangements are explained by the following categories;
1 = A resit is available for all components of the module
2 = No resit is available for 100% continuous assessment module
3 = No resit is available for the continuous assessment component
This module is category 2
Indicative Reading List

    Other Resources

    Programme or List of Programmes
    CHPMMaster of Science

    My DCU | Loop | Disclaimer | Privacy Statement