Module: Bioanalytical Practicals II

Latest Module Specifications

Current Academic Year 2025 - 2026

Module Title	Bioanalytical Practicals II
Module Code	BIO1015
Faculty	Biotechnology	School	Science & Health
NFQ level	8	Credit Rating	5

Description

This module offers hands-on training in selected cell biology, immunology, and molecular biology techniques to reinforce understanding of key concepts in these areas.

Learning Outcomes

1. Demonstrate knowledge and comprehension of the basic principles and applications of main bioanalytical techniques, including cell culture, immunoassays, isolation, and analysis of deoxyribonucleic acid (DNA).
2. Perform a range of bioanalytical techniques, such as basic cell culture, cell viability assay (MTS), enzyme-linked immunosorbent assay (ELISA), sodium dodecyl-sulphate polyacrylamide gel electrophoresis (SDS-PAGE), Western Blotting, chromatographic separation and purification of nucleic acids, DNA quantification using spectrophotometry, polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (PCR-RFLP) ensuring proper use of instruments and adherence to safety protocols.
3. Critically analyse and interpret experimental data obtained from bioanalytical experiments. Identify and resolve common issues that arise during bioanalytical procedures, including instrument malfunction, sample contamination, and data anomalies.
4. Collaborate effectively in teams to plan, execute, and report on bioanalytical experiments, demonstrating communication, teamwork, and time management skills.
5. Communicate the scientific results of bioanalytical experiments through in-class discussions and written lab reports, using appropriate scientific terminology and data visualisation techniques.

*Type*	*Hours*	*Description*
Workload	Full time hours per semester
Laboratory	48	Laboratory work and engagement with pre-recorded material
Directed learning	50	Preparation for assessment
Independent Study	27	Data interpretation and report writing
Total Workload: 125

Section Breakdown
CRN	10158	Part of Term	Semester 1
Coursework	0%	Examination Weight	0%
Grade Scale	40PASS	Pass Both Elements	Y
Resit Category	RC2	Best Mark	N
Module Co-ordinator	Jianghui Meng	Module Teacher	Christine Loscher, Denise Harold, Paul Cahill, Tomas Zurawski

Type	Description	% of total	Assessment Date
Assessment Breakdown
In Class Test	Two in-class tests will evaluate the acquired knowledge of terminology, basic concepts and data interpretation on the topics covered. (2 X 20% = 40% of final mark)	40%	n/a
Laboratory Portfolio	Two experimental write-ups will assess understanding of the purpose of each experiment, its design, data collection and analysis, and interpretation of the results within the context of the relevant scientific concepts. Students will be assessed on their understanding of experimental procedures, data accuracy and analysis, critical thinking, scientific communication and the clarity of their conclusions. (2 x 30% = 60% of final mark)	60%	n/a

Reassessment Requirement Type
Resit arrangements are explained by the following categories; RC1: A resit is available for both^* components of the module. RC2: No resit is available for a 100% coursework module. RC3: No resit is available for the coursework component where there is a coursework and summative examination element. ^* ‘Both’ is used in the context of the module having a coursework/summative examination split; where the module is 100% coursework, there will also be a resit of the assessment

Pre-requisite	None
Co-requisite	None
Compatibles	None
Incompatibles	None

All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml

Indicative Content and Learning Activities

Basic cell culture techniques.

Detection of a selected biomarker by enzyme-linked immunosorbent assay (ELISA).

Cell lysis and protein extraction for gel electrophoresis (SDS-PAGE) and immunodetection (Western Blotting).

Preparation and analysis of mammalian DNA.

Single nucleotide polymorphism (SNP) characterisation by PCR-restriction fragment length polymorphism (PCR-RFLP).

SNP detection by PCR using fluorescent hybridisation probes and melting curve analysis.

Indicative Reading List

Books:
None

Articles:
None

Other Resources

None