Registry
Module Specifications
Archived Version 2005 - 2006
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Module Aims
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7 To provide a detailed working knowledge of methods used in advanced immunoanalysis - their applications, advantages and limitations.
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Learning Outcomes
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Students should gain:
Practical experience of the production of monoclonal antibodies.
Detailed knowledge of the production and characterisation of antibodies and enzyme-labelled antibodies.
'Hands-on' experience of advanced aspects of immunoanalysis.
Experience in mixing theoretical and practical skills.
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Indicative Time Allowances
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Hours
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Lectures |
1
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Tutorials |
0
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Laboratories |
50
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Seminars |
0
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Independent Learning Time |
24
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Total |
75
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Placements |
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Assignments |
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NOTE
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Assume that a 5 credit module load represents approximately 75 hours' work, which includes all teaching, in-course assignments, laboratory work or other specialised training and an estimated private learning time associated with the module.
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Indicative Syllabus
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¿ Production of monoclonal antibodies. Students will perform and monitor a myeloma/spleen fusion.
¿ Isolation of specific antibodies using salt precipitation and affinity chromatography.
¿ Antibody characterisation including SDS electrophoresis, HPLC and ELISA.
¿ Production and characterisation of antibody-enzyme conjugates.
¿ Use of the Biacore 'Real-Time' Sensor system in immunoanalysis.
¿ Immunodiagnostic methods, including immunoblotting techniques.
¿ Immunofluroescence techniques.
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| Assessment | | Continuous Assessment | 100% | Examination Weight | 0% |
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Indicative Reading List
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Essential:
1. Practical Manual provided for course.
Supplementary:
1. Practical Immunology by Hudson & Hay (Blackwell,1994).
2. Enzyme Immunoassay by P. Tijssen (Elsevier, 1985).
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Programme or List of Programmes
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| GDBL | Graduate Diploma in Biological Sciences |
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