Module Title |
Separation Techniques
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Module Code |
CS302
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School |
School of Chemical Sciences
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Online Module Resources
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Level |
3
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Credit Rating |
5
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Pre-requisite |
None
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Co-requisite |
None
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Module Aims
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To introduce the student to the theory and practices of modern separation techniques.
To provide the necessary background to allow the student to select an appropriate separation technique for a given analysis.
To introduce the student to the application of chromatography in a wide range of environments e.g. industrial, pharmaceutical, environmental.
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Learning Outcomes
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The student should be able to:
7 Understand the thermodynamic and molecular basis for equilibrium separation.
7 Calculate and measure tr, k, a, Rs, N and H for a given separation.
7 Explain the individual and collective contributions to bond broadening and ways in which they can be minimised.
7 Describe the sample preparation, method development and instrumentation required for gas chromatography, high performance liquid chromatography, ion chromatography, capillary electrophoresis and other separation techniques.
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Indicative Time Allowances
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Hours
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Lectures |
30
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Tutorials |
6
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Laboratories |
0
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Seminars |
0
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Independent Learning Time |
39
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Total |
75
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Placements |
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Assignments |
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NOTE
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Assume that a 5 credit module load represents approximately 75 hours' work, which includes all teaching, in-course assignments, laboratory work or other specialised training and an estimated private learning time associated with the module.
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Indicative Syllabus
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1. Chemical equilibria; activity, acid-base chemistry, formation constants, solubility products.
2. Thermodynamics of separation - distribution coefficients, chemical potential and conditions for equilibrium, solvent extractions.
3. Molecular basis for equilibrium separation - the effect of molecular structure on the distribution of a compound between two phases. The empirical approach - Rohrschneider constant.
4. Chromatography separation - definition and classification of chromatographic mechanisms, sorption isotherms, retention behaviour, distribution coefficients, capacity factor, column selectivity, column efficiency, bond broadening, diffusion, mass transfer, resolution, quantitative methods.
5. Sample preparation - solid phase extraction and derivatisation.
6. Gas chromatography - principles, stationary phases and instrumentation.
7. High-performance liquid chromatography (HPLC) – classification, basic LC concepts (k ,a, R and N), instrumentation used (pumps, injectors, columns, detectors), chromatographic modes (reversed-phase, normal-phase, ion-exchange, size-exclusion, affinity) and applications.
8. Capillary electrophoresis (CE) - classification, basic CE concepts (m, v, EOF, N and R), instrumentation used (power supply, injectors, capillaries, detectors), CE modes (CZE, CGE, MECC, CEC, chiral CE).
9. Chiral chromatography
10. Ion chromatography.
11. Other separation techniques including LC-MS.
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Assessment | Continuous Assessment | 0% | Examination Weight | 100% |
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Indicative Reading List
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Essential:
Recommended:
Quantitative chemical analysis 4th and 5th editions – D.C. Harris.
Chromatography and Separation Science, Vol. 4 in Separation Science and Technology Series, S. Ahuja, 2002, Academic Press, ISBN: 0120449811. DCU library ref.: 543.089
HPLC and CE – Principles and Practice, A. Weston and P. Brown, 1997, Academic Press, ISBN: 0121366405. DCU library ref.: 543.0871
Instrumental Methods of Analysis, Willard, Merritt, Dean & Settle - Wadswarlt 1988
Chromatography, 5th edition, fundamentals and techniques. Ed. E.Heftmann.
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Programme or List of Programmes
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AC | BSc in Chemical and Pharmaceutical Sc. |
AS | BSc in Analytical Science |
BSSA | Study Abroad (DCU Business School) |
BSSAO | Study Abroad (DCU Business School) |
CF | BSc in Chemistry with French |
CG | BSc in Chemistry with German |
ECSA | Study Abroad (Engineering & Computing) |
ECSAO | Study Abroad (Engineering & Computing) |
HMSA | Study Abroad (Humanities & Soc Science) |
HMSAO | Study Abroad (Humanities & Soc Science) |
SHSA | Study Abroad (Science & Health) |
SHSAO | Study Abroad (Science & Health) |
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