Registry
Module Specifications
Archived Version 2007 - 2008
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Module Aims | |||||||||||||||||||||||||||||
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Learning Outcomes | |||||||||||||||||||||||||||||
Students will be able to understand:
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Assume that a 5 credit module load represents approximately 75 hours' work, which includes all teaching, in-course assignments, laboratory work or other specialised training and an estimated private learning time associated with the module. | |||||||||||||||||||||||||||||
Indicative Syllabus | |||||||||||||||||||||||||||||
Recombinant DNA technology - restriction enzymes, joining DNA molecules. DNA amplification, the polymerase chain reaction. Vectors for gene cloningPlasmids, introduction to plasmids - structure, high and low copy number, different forms, plasmid encoded phenotypes.Replication of plasmids -direction of replication, priming, elongation, termination, control of replication, host functions, incompatibility, amplification.Bacteriophages - phage lambda, lytic and lysogenic life cycle, replication of lambda, packaging, control of lysogeny. Cosmid and phagemid vectors for gene cloning.Introduction of DNA into cells, gene libraries, identification of recombinant clones.DNA analysis, hybridisation, Southern and northern blotting, DNA synthesis DNA sequencing methodologies.
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Indicative Reading List | |||||||||||||||||||||||||||||
Essential: Molecular Genetics of Bacteria, J. Dale (Wiley).Analysis of Genes and Genomes, R.J. ReeceGenetics - a Molecular Approach, T.A. Brown, 2nd Edition (Chapman & Hall). Recombinant DNA, Watson, Gilman, Witkowski and Zoller (Scientific AmericanBooks, 1992). Supplementary: Bacterial Plasmids, K. Hardy (van Nostrand).Human Molecular Genetics, Strachan and Read (BIOS Scientific, 2000).An introduction to GENETIC ANALYSIS, Griffiths et al. | |||||||||||||||||||||||||||||
Programme or List of Programmes | |||||||||||||||||||||||||||||
AS | BSc in Analytical Science | ||||||||||||||||||||||||||||
BSSA | Study Abroad (DCU Business School) | ||||||||||||||||||||||||||||
BSSAO | Study Abroad (DCU Business School) | ||||||||||||||||||||||||||||
BT | BSc in Biotechnology | ||||||||||||||||||||||||||||
ECSA | Study Abroad (Engineering & Computing) | ||||||||||||||||||||||||||||
ECSAO | Study Abroad (Engineering & Computing) | ||||||||||||||||||||||||||||
GCB | BSc in Genetics & Cell Biology | ||||||||||||||||||||||||||||
HMSA | Study Abroad (Humanities & Soc Science) | ||||||||||||||||||||||||||||
HMSAO | Study Abroad (Humanities & Soc Science) | ||||||||||||||||||||||||||||
SHSA | Study Abroad (Science & Health) | ||||||||||||||||||||||||||||
SHSAO | Study Abroad (Science & Health) | ||||||||||||||||||||||||||||
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