Registry
Module Specifications
Archived Version 2007 - 2008
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Module Title |
Bioinformatics and Gene Cloning
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Module Code |
BE380
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School |
School of Biotechnology
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Online Module Resources
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Level |
3
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Credit Rating |
5
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Pre-requisite |
None
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Co-requisite |
None
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Module Aims
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- To introduce students to applications of Web based bioinformatic analyses
- To train students in the basic practical techniques of recombinant DNA technology and to develop their understanding of strategies used in the construction, mapping and expression of recombinant molecules.
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Learning Outcomes
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Students should have the technical ability to:
- Construct, analyse and map recombinant plasmid molecules.
- Use some of the basic bioinformatics package to find and analyse gene sequences.
Students should have developed an understanding of:
- Different strategies in the construction and mapping of recombinant molecules.
- The role of bioinformatics in the identification and analysis of gene sequences.
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Indicative Time Allowances
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Hours
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Lectures |
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Tutorials |
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Laboratories |
72
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Seminars |
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Independent Learning Time |
3
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Total |
75
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Placements |
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Assignments |
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NOTE
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Assume that a 5 credit module load represents approximately 75 hours' work, which includes all teaching, in-course assignments, laboratory work or other specialised training and an estimated private learning time associated with the module.
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Indicative Syllabus
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Overview of bioinformatics and nucleotide and protein databases. Derivation and format of nucleotide and protein sequences e.g. FASTA. Using web resources e.g. NCBI to access and retrieve sequences. Comparing DNA and protein sequences with appropriate databases using BLAST. Displaying similarities using alignments and multiple alignmentsIdentification of coding regions and consensus sequences. Translating nucleotide sequences and searching for open reading frames. Analysis of nucleotide sequences for restriction enzyme sites. Primer design for the PCR. Application of PCR for the amplification of target genes. Analysis of DNA by agarose gel electrophoresis. DNA restriction and restriction mappingCloning in plasmid vectorsLigation. Transformation and electroporation. Extraction of plasmid DNA from transformants and its analysis by restriction and gel electrophoresis. Site directed mutagenesis.
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| Assessment | | Continuous Assessment | 100% | Examination Weight | 0% |
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Indicative Reading List
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Programme or List of Programmes
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| GCB | BSc in Genetics & Cell Biology |
| Archives: | |
