Registry
Module Specifications
Archived Version 2007 - 2008
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| Module Aims | |||||||||||||||||||||||||||||||||
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The aim of this module is to
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| Learning Outcomes | |||||||||||||||||||||||||||||||||
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1. Understand the principles of protein chemistry in solution. 2. Design a DSP process which is readily integrated with the USP 3. Decide how to choose the correct separation and chromatographic techniques. 4. Understand the theory and practice of chromatography and how this can help in designing the DSP. 5. Comprehend the different types of chromatographic equipment and how they operate | |||||||||||||||||||||||||||||||||
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| NOTE | |||||||||||||||||||||||||||||||||
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Assume that a 5 credit module load represents approximately 75 hours' work, which includes all teaching, in-course assignments, laboratory work or other specialised training and an estimated private learning time associated with the module. | |||||||||||||||||||||||||||||||||
| Indicative Syllabus | |||||||||||||||||||||||||||||||||
| This module is built on the fundamental concepts of protein structure and function, physical chemistry of solutions and electrolytes/ acids/bases and pH to understand how proteins behave in solution and how their properties may be altered by changing the physical surroundings. Using examples, descriptions will be given as to how to isolate proteins from inclusion bodies or culture medium using a range of chemical and physical separation processes. The principles of developing a purification process, including theory, will be defined using a wide range of chromatographic techniques including, but not exclusive to; gel filtration, ion-exchange, hydrophobic interaction, reverse phase, metal chelate, immunoaffinity and other affinity- based techniques, microfiltration and others. The different types of equipment, fluidized versus packed- beds and how these may be scaled- up will be discussed. | |||||||||||||||||||||||||||||||||
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| Indicative Reading List | |||||||||||||||||||||||||||||||||
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Protein Purification – Principles & Practice – Robert K. Scopes 3rd Edition (1994)/Springer Verlag Bioseparations Downstream Processing for Biotechnology – Paul Belter, E.L. Cussler, Wei-Shou Hu - (1988)/John Wiley & Sons Lecturers Notes | |||||||||||||||||||||||||||||||||
| Programme or List of Programmes | |||||||||||||||||||||||||||||||||
| SMPSC | Single Module Professional Science | ||||||||||||||||||||||||||||||||
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