Registry
Module Specifications
Archived Version 2009 - 2010
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Module Title |
Bioanalytical Practicals II
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Module Code |
BE452
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School |
School of Biotechnology
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Online Module Resources
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| Module Co-ordinator | Dr Dermot Walls | Office Number | X217 |
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Level |
4
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Credit Rating |
5
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Pre-requisite |
None
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Co-requisite |
None
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Module Aims
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- To provide skills in cell biology and biochemistry
- To teach and discuss methods in microbiological techniques.
- To show the use of genetic and microbiological analytical method
- To teach immunological analysis methods and their applications.
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Learning Outcomes
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Students should gain :·
- A knowledge of cell culture, electrophoretic, radioisotopic and chromatographic methods·
- An understanding of microorganism preservation, disinfectant evaluation, innoculum development, and activated sludge estimation ·
- An overview of the methods of the generation of DNA probes, Southern blotting and hybridisation methods, and microbiological assays for antibiotics and mutagens·
- A knowledge of the isolation and characterisation of polyclonal antibodies, conjugation of antibodies with enzyme labels, and immunoanalytical methods.
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Indicative Time Allowances
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Hours
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Lectures |
0
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Tutorials |
0
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Laboratories |
72
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Seminars |
10
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Independent Learning Time |
-7
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Total |
75
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Placements |
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Assignments |
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NOTE
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Assume that a 5 credit module load represents approximately 75 hours' work, which includes all teaching, in-course assignments, laboratory work or other specialised training and an estimated private learning time associated with the module.
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Indicative Syllabus
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The students perform cell culture methods, electrophoresis, anion and cation exchange chromatography and radioisotopic assays.
- The microbial organisms in activated sludge will be estimated, and methods of innoculum development and microorganism preservation performed.
- Southern blotting and hybridisation techniques with non-radioactive probes will be carried out· Immunoglobulins will be isolated from animal serum, chemically conjugated to enzymes and used in enzyme linked immunosorbant assays
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| Assessment | | Continuous Assessment | 100% | Examination Weight | 0% |
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Indicative Reading List
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Essential:Cell Culture, Microbiology, Immunology and Genetics Practical Manuals, DCU
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Programme or List of Programmes
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| AS | BSc in Analytical Science |
| Archives: | |
