Registry
Module Specifications
Archived Version 2009 - 2010
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Module Title |
Advanced Practical Immunology and Immunoanalysis
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Module Code |
BE553
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School |
School of Biotechnology
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Online Module Resources
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| Module Co-ordinator | Dr. Paul Leonard | Office Number | A210 |
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Level |
5
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Credit Rating |
5
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Pre-requisite |
None
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Co-requisite |
None
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Module Aims
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7 To provide a detailed working knowledge of methods used in advanced immunoanalysis - their applications, advantages and limitations.
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Learning Outcomes
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Students should gain: Practical experience of the production of monoclonal antibodies. Detailed knowledge of the production and characterisation of antibodies and enzyme-labelled antibodies. 'Hands-on' experience of advanced aspects of immunoanalysis. Experience in mixing theoretical and practical skills.
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Indicative Time Allowances
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Hours
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Lectures |
1
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Tutorials |
0
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Laboratories |
50
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Seminars |
0
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Independent Learning Time |
24
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Total |
75
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Placements |
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Assignments |
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NOTE
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Assume that a 5 credit module load represents approximately 75 hours' work, which includes all teaching, in-course assignments, laboratory work or other specialised training and an estimated private learning time associated with the module.
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Indicative Syllabus
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¿ Production of monoclonal antibodies. Students will perform and monitor a myeloma/spleen fusion.¿ Isolation of specific antibodies using salt precipitation and affinity chromatography.¿ Antibody characterisation including SDS electrophoresis, HPLC and ELISA.¿ Production and characterisation of antibody-enzyme conjugates.¿ Use of the Biacore ''Real-Time'' Sensor system in immunoanalysis.¿ Immunodiagnostic methods, including immunoblotting techniques.¿ Immunofluroescence techniques.
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| Assessment | | Continuous Assessment | 100% | Examination Weight | 0% |
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Indicative Reading List
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Essential: 1. Practical Manual provided for course. Supplementary:1. Practical Immunology by Hudson & Hay (Blackwell,1994).2. Enzyme Immunoassay by P. Tijssen (Elsevier, 1985).
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Programme or List of Programmes
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| GBD | Grad. Diploma in Biomedical Diagnostics |
| MBD | MSc in Biomedical Diagnostics |
| Archives: | |
