Module Title |
Separation Techniques
|
Module Code |
CS302
|
School |
School of Chemical Sciences
|
Online Module Resources
|
Module Co-ordinator | Prof Fiona Regan | Office Number | X116A |
Level |
3
|
Credit Rating |
5
|
Pre-requisite |
None
|
Co-requisite |
None
|
|
Module Aims
|
- To introduce the student to the theory and practices of modern separation techniques.
- To provide the necessary background to allow the student to select an appropriate separation technique for a given analysis.
- To introduce the student to the application of chromatography in a wide range of environments e.g. industrial, pharmaceutical, environmental.
|
Learning Outcomes
|
The student should be able to:
- Understand the thermodynamic and molecular basis for equilibrium separation. Calculate and measure tr, k, a, R, N and H for a given separation. Explain the individual and collective contributions to band broadening and ways in which they can be minimised
- Describe the sample preparation, method development parameters and instrumentation required for (i) gas chromatography, (ii) high performance liquid chromatography (iii) ion chromatography, (iv) capillary electrophoresis and other separation techniques.
|
Indicative Time Allowances
|
|
Hours
|
Lectures |
30
|
Tutorials |
6
|
Laboratories |
0
|
Seminars |
0
|
Independent Learning Time |
39
|
|
|
Total |
75
|
Placements |
|
Assignments |
|
|
NOTE
|
Assume that a 5 credit module load represents approximately 75 hours' work, which includes all teaching, in-course assignments, laboratory work or other specialised training and an estimated private learning time associated with the module.
|
Indicative Syllabus
|
- Chemical equilibria; activity, acid-base chemistry, formation constants, solubility products.
- Thermodynamics of separation - distribution coefficients, chemical potential and conditions for equilibrium, solvent extractions.
- Molecular basis for equilibrium separation - the effect of molecular structure on the distribution of a compound between two phases. The empirical approach - Rohrschneider constant.
- Chromatography separation - definition and classification of chromatographic mechanisms, sorption isotherms, retention behaviour, distribution efficients, capacity factor, column selectivity, column efficiency, bond broadening, diffusion, mass transfer, resolution, quantitative methods.
- Sample preparation - solid phase extraction and derivatisation.
- Gas chromatography - principles, stationary phases and instrumentation.
- High-performance liquid chromatography (HPLC) – classification, basic LC concepts (k , a, R and N), instrumentation used (pumps, injectors, columns, detectors), chromatographic modes (reversed-phase, normal-phase, ion-exchange, size-exclusion, affinity) and applications.
- Capillary electrophoresis (CE) - classification, basic CE concepts (m, v, EOF, N and R), instrumentation used (power supply, injectors, capillaries, detectors), CE modes (CZE, CGE, MECC, CEC, chiral CE).
- Chiral chromatography and Ion chromatography.
- Other separation techniques including LC-MS.
|
Assessment | Continuous Assessment | 0% | Examination Weight | 100% |
|
Indicative Reading List
|
Recommended:
- Quantitative chemical analysis 4th and 5th editions – D.C. Harris.
- Chromatography and Separation Science, Vol. 4 in Separation Science and Technology Series, S. Ahuja, 2002, Academic Press, ISBN: 0120449811. DCU library ref.: 543.089
- HPLC and CE – Principles and Practice, A. Weston and P. Brown, 1997, Academic Press, ISBN: 0121366405. DCU library ref.: 543.0871
- Instrumental Methods of Analysis, Willard, Merritt, Dean & Settle - Wadswarlt 1988 Chromatography, 5th edition, fundamentals and techniques. Ed. E.Heftmann.
|
|
Programme or List of Programmes
|
AC | BSc in Chemical and Pharmaceutical Sc. |
AS | BSc in Analytical Science |
BSSA | Study Abroad (DCU Business School) |
BSSAO | Study Abroad (DCU Business School) |
CG | BSc in Chemistry with German |
ECSA | Study Abroad (Engineering & Computing) |
ECSAO | Study Abroad (Engineering & Computing) |
HMSA | Study Abroad (Humanities & Soc Science) |
HMSAO | Study Abroad (Humanities & Soc Science) |
SHSA | Study Abroad (Science & Health) |
SHSAO | Study Abroad (Science & Health) |
Archives: | |