Registry
Module Specifications
Archived Version 2014 - 2015
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Description The purpose of this module is to develop and articulate a systematic understanding of knowledge of the biology of recombinant DNA cloning and control of gene expression, or informed by, the forefront of this field. This will include the development of systematic knowledge underpinning specialised research tools and techniques used in the manipulation of DNA including relevant qualitative and quantitative theoretical background that would prepare students to acquire and demonstrate the skills involved in DNA (and RNA) manipulation. To develop and articulate a systematic understanding of knowledge of the complexity of gene expression and its control, with an emphasis on eukaryotic systems at, or informed by, the forefront of this field. | |||||||||||||||||||||||||||||||||||||
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Learning Outcomes 1. Develop and articulate an understanding of knowledge at the forefront of research both qualitatively, and, where appropriate, quantitatively, around the underpinning science of the design of DNA cloning strategies exploiting the knowledge of restriction enzymes, vector systems and PCR technology 2. Demonstrate specialised research tools and techniques by analysing genome sequences to gain an understanding of the genetic organisation of the encoded information. 3. Demonstrate specialised research tools and techniques by designing an experimental approach to monitor the expression of genes using RNA analysis 4. Demonstrate specialised research tools and techniques by selecting the appropriate vector system for application in genetic analysis 5. Demonstrate specialised research tools and techniques and also a critical awareness of current problems/new insights, informed by the forefront of learning in the field, by applying the knowledge gained in the design of specific experiments towards their research project aims and objectives. | |||||||||||||||||||||||||||||||||||||
All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml |
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Indicative Content and
Learning Activities Recombinant DNA technology: Conventional and contemporary methods of cloning such as Gateway cloning. Introduction to cloning vectors, restriction enzymes, plasmids, ligations. Replication of plasmids.DNA amplification. Polymerase Chain Reaction and its various applications in the amplification of DNA, cDNA. RNA, reverse transcription and quantitation. Chromatin immunoprecipitation, mobility shift assays. Sanger sequencing and its applications.Eukaryotic and prokaryotic gene expression. Prokaryotic: operons and coordinated gene control. Eukaryotic: promoters and various levels of control. Splicing. miRNAs. Chromatin.Human Genome Content. Overview of the human genome and the types of genes and functional elements | |||||||||||||||||||||||||||||||||||||
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Indicative Reading List
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Other Resources None | |||||||||||||||||||||||||||||||||||||
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