Registry
Module Specifications
Archived Version 2015 - 2016
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Description The purpose of this module is to introduce students to the growing of animal cells in culture, the important parameters to be considered in the cultivation of animal cells in vitro, good laboratory procedures in the handling and growing of animal cells, and the scale up of cells. Students are expected to attend lectures. | |||||||||||||||||||||||||||||||||||||
Learning Outcomes 1. Have an ability to critically analyse the importance of aseptic techniques and the technology needed to implement this. 2. Assess the Q.A. issues that effect animal cell culture and implement the good laboratory practice for the correct handling of animal cells. 3. Have an ability to assess the types of cell cultures to use for specific studies. 4. Evaluate the growth media for animal cells, its constituents, the growth factors added, and how it influences cell behavior. 5. Evaluate and select the optimum methods and cell based assays to characterize cells and study different process 6. Critically analyse the problems and the processes required in scaling up animal cell cultures. | |||||||||||||||||||||||||||||||||||||
All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml |
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Indicative Content and
Learning Activities Cell Culture TechniqueCulturing animal cells; aseptic technique; cyropreservation; sub cultivation; media formulation and culture environment.Sterility & Aseptic TechniquesTypes of microbial contamination; Methods of detection.Primary Cell CultureTechniques used to isolate and grow cells in vitro; The importance of culture conditions in the growth of a selected cell type.Cell Based AssaysBasics of proliferation assays; Different proliferation assay protocols; Basics of Invasion and migration assays; Cell adhesion assays and In vitro models of disease.Cell IdentificationThe importance of cell identification; methods to authenticate cells. | |||||||||||||||||||||||||||||||||||||
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Indicative Reading List
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Other Resources None | |||||||||||||||||||||||||||||||||||||
Programme or List of Programmes | |||||||||||||||||||||||||||||||||||||
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