Registry
Module Specifications
Archived Version 2017 - 2018
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Description To introduce the student to the theory and practices of modern separation techniques. To provide the necessary background to allow the student to select an appropriate separation technique for a given analysis. To introduce the student to the application of chromatography in a wide range of environments e.g. industrial, pharmaceutical, environmental. | |||||||||||||||||||||||||||||||||||||
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Learning Outcomes 1. Understand the thermodynamic and molecular basis for equilibrium separation. 2. Calculate and measure tr, k, a, R, N and H for a given separation. 3. Explain the individual and collective contributions to band broadening and ways in which they can be minimised 4. Describe the sample preparation, method development parameters and instrumentation required for (i) gas chromatography, (ii) high performance liquid chromatography (iii) ion chromatography, (iv) capillary electrophoresis and other separation techniques. | |||||||||||||||||||||||||||||||||||||
All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml |
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Indicative Content and
Learning Activities Chemical EquilibriaChemical equilibria; activity, acid-base chemistry, formation constants, solubility productsThermodynamics of separationdistribution coefficients, chemical potential and conditions for equilibrium, solvent extractions.Molecular basis for equilibrium separationthe effect of molecular structure on the distribution of a compound between two phasesChromatography separationdefinition and classification of chromatographic mechanisms, sorption isotherms, retention behaviour, distribution co-efficients, capacity factor, column selectivity, column efficiency, bond broadening, diffusion, mass transfer, resolution, quantitative methodsSample preparationsolid phase extraction and derivatisationGas chromatographyprinciples, stationary phases and instrumentationHigh-performance liquid chromatography (HPLC)classification, basic LC concepts (k , a, R and N), instrumentation used (pumps, injectors, columns, detectors), chromatographic modes (reversed-phase, normal-phase, ion-exchange, size-exclusion, affinity) and applicationsCapillary electrophoresis (CE)classification, basic CE concepts (m, v, EOF, N and R), instrumentation used (power supply, injectors, capillaries, detectors), CE modes (CZE, CGE, MECC, CEC, chiral CE)Chiral chromatography and Ion chromatographyOther separation techniques including LC-MS | |||||||||||||||||||||||||||||||||||||
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Indicative Reading List
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Other Resources None | |||||||||||||||||||||||||||||||||||||
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