Registry
Module Specifications
Archived Version 2019 - 2020
| |||||||||||||||||||||||||||||||||||||
|
Description To develop a good understanding of the biology of bacteriophages and plasmids. To teach the basis for techiques used in the manipulation of DNA and to provide a theoretical background that would enable students to learn the skills involved in DNA manipulation. To develop an appreciation and understanding of the complexity of gene expression and its control, with an emphasis on eukaryotic systems. | |||||||||||||||||||||||||||||||||||||
|
Learning Outcomes 1. Design DNA cloning strategies exploiting the knowledge of restriction enzymes, vector systems and PCR technology 2. Analyse genome sequences to gain an understanding of the genetic organisation of the encoded information 3. Design an experimental approach to monitor the expression of genes using RNA analysis 4. Select the appropriate vector system for application in genetic analysis | |||||||||||||||||||||||||||||||||||||
All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml |
|||||||||||||||||||||||||||||||||||||
|
Indicative Content and
Learning Activities Recombinant DNA technology- restriction enzymes, joining DNA molecules. DNA amplification, the polymerase chain reaction. Vectors for gene cloningPlasmids, introduction to plasmids- structure, high and low copy number, different forms, plasmid encoded phenotypes.Replication of plasmidsDNA sequencing methodologies. | |||||||||||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||||||||||
Indicative Reading List
| |||||||||||||||||||||||||||||||||||||
|
Other Resources None | |||||||||||||||||||||||||||||||||||||
| Programme or List of Programmes | |||||||||||||||||||||||||||||||||||||
| Archives: |
| ||||||||||||||||||||||||||||||||||||