Registry
Module Specifications
Archived Version 2019 - 2020
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Description This module will provide a detailed understanding of the methods used in: (i) mammalian cell biology including basic cell culture techniques, cell counting, seeding growth and cell viability studies in addition to training in techniques for assessment of cell differentiation (phenotype) in situ (immunocytochemistry), cell signalling and protein expression (Western blotting) and cell secretion of proteins (ELISA-enzyme-linked immunosorbent assay). (ii) The basic practical techniques of Polymerase Chain Reaction, recombinant DNA technology and to develop their understanding of strategies used in the construction, confirmation and expression of recombinant molecules in bacterial cells. Bioinformatics will include sequence retrieval and analysis of protein coding sequences. Attendance at practical sessions is mandatory and students are expected to engage in tutorial sessions and complete assignments. | |||||||||||||||||||||||||||||||||||||
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Learning Outcomes 1. Perform routine cell culture using mammalian adherent adult stem cells to learn basic cell counting, cell adherence growth and viability assays. 2. Measure the phenotype and differentiation state of a cell using fluorescent immunocytochemistry in situ. 3. Measure the concentration of an intracellular protein in a cell lysate. 4. Perform SDS-PAGE gel electrophoresis and Western blot analysis to prove for the presence of specific intracellular proteins. 5. Perform an Enzyme Linked Immunosorbent Assay (ELISA) of secreted proteins from cells. 6. Perform basic recombinant DNA cloning techniques. 7. Use specific genomic databases and bioinformatics software packages. 8. Design an experiment to generate a recombinant DNA clone of a gene of interest using PCR. 9. Interpret restriction maps of recombinant DNA molecules. | |||||||||||||||||||||||||||||||||||||
All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml |
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Indicative Content and
Learning Activities Mammalian cell culture and assessment of growth and viability.The techniques of fluorescent in situ immunocytochemistry, protein concentration determination, Western blotting & ELISARecombinant DNA cloning in E.coliRecombinant DNA cloning in E.coli and includes the following techniques: PCR, gel electrophoresis, Gateway cloning technology, bacterial transformation and selection, restriction enzyme mapping.Access and analyse a specific protein coding DNA sequence of interest. | |||||||||||||||||||||||||||||||||||||
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Indicative Reading List | |||||||||||||||||||||||||||||||||||||
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Other Resources 35541, 0, Lab manuals and associated articles and resources available on Loop., | |||||||||||||||||||||||||||||||||||||
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