Registry
Module Specifications
Archived Version 2021 - 2022
| |||||||||||||||||||||||||||||||||||||
|
Description Module aims: This module will give the student an understanding of how recombinant DNA technologies are used to manipulate both cloned genes and host producer cells so that biopharmaceuticals and diagnostic reagents can be safely and stably produced using appropriate cell-based systems. | |||||||||||||||||||||||||||||||||||||
|
Learning Outcomes 1. Select from complex issues in protein engineering and demonstrate a systematic understanding of how these may be addressed whereby recombinant genes are manipulated leading to the generation of recombinant proteins with modified properties. 2. Demonstrate a range of standard and specialised recombinant DNA expression systems and tools that are designed to ensure high-level production of soluble, stable, purifiable and authentic recombinant proteins. 3. Exhibit a critical awareness of genome engineering and gene knockdown technologies and give insights into their applications in producer cell engineering and gene therapy. 4. Exercise initiative and a capacity for independent research by using contemporary techniques of enquiry to critically appraise relevant examples of products and methods from the forefront of the Biopharma and Diagnostic fields. | |||||||||||||||||||||||||||||||||||||
All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml |
|||||||||||||||||||||||||||||||||||||
|
Indicative Content and
Learning Activities Indicative ContentOverview of the structure and roles of DNA, RNA and protein molecules; Genomes, genome sequencing and molecular biology methods including PCR; Cloning and manipulating DNA sequences; Vectors and hosts for expressing recombinant proteins; Transfection and selection of recombinant cells; Protein engineering strategies -case studies; Antibody engineering strategies -case studies; RNA interference/gene knockdown; Bioanalysis of biologics for nucleic acid content | |||||||||||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||||||||||
|
Indicative Reading List | |||||||||||||||||||||||||||||||||||||
|
Other Resources None | |||||||||||||||||||||||||||||||||||||
| Programme or List of Programmes | |||||||||||||||||||||||||||||||||||||
| Archives: |
| ||||||||||||||||||||||||||||||||||||