Registry
Module Specifications
Archived Version 2022 - 2023
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Description To develop a good understanding of the biology of bacteriophages and plasmids. To teach the basis of techniques used in the manipulation of DNA/RNA and to provide a theoretical background that would enable students to learn the skills involved in nucleic acid manipulation. To develop an appreciation and understanding of the complexity of gene expression and its control, with an emphasis on eukaryotic systems. To give an overview of the content of Eukaryotic genomes and their functionality. | |||||||||||||||||||||||||||||||||||||
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Learning Outcomes 1. Design and understand recombinant DNA cloning strategies and other relevant molecular biological methodologies useful in the investigation of nucleic acids. 2. Select the appropriate vector system for application in genetic analysis 3. Analyse genome sequences to gain an understanding of gene organisation and function of the encoded information- a focus on the gene itself as a functional unit 4. Design an experimental approach to monitor the expression of genes using RNA analysis 5. Gain knowledge and understanding of the complexity of Eukaryotic Gene Expression control. | |||||||||||||||||||||||||||||||||||||
All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml |
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Indicative Content and
Learning Activities Recombinant DNA technology- restriction enzymes, joining DNA molecules. DNA amplification, the polymerase chain reaction. Vectors for gene cloningPlasmids, introduction to plasmids- structure, high and low copy number, different forms, plasmid encoded phenotypes.Replication of plasmidsDNA sequencing methodologies. | |||||||||||||||||||||||||||||||||||||
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Indicative Reading List
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Other Resources None | |||||||||||||||||||||||||||||||||||||
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