Registry
Module Specifications
Archived Version 2023 - 2024
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Description Module Aims: The purpose of this module is to prepare the students in practical laboratory techniques that are relevant to the field of biotherapeutics. The students will develop a good understanding of the theory and practice of a number of practical techniques including understanding how to perform basic techniques in molecular biology such as gene cloning, animal cell culture and cell viability assays, transfection, ELISA, and Flow cytometry. The student will develop a good understanding of the theory and practice of the biomedical techniques and be able to critically analyse and interpret data to produce biotherapeutics for diseases such as cancer, chronic inflammatory diseases, nervous system or cardiovascular system diseases. Module Outcomes: LO1 To gain a systematic understanding of the theory and practice of the following practical techniques: 1. Animal Cell Culture, 2. Cell Viability Assays, 3. ELISA, 4. Flow cytometry, 5. Transfection and 6. Gene cloning. LO2 To gain critical awareness of data analysis and presentation in a professional manner. LO3 To acquire specialised tools and knowledge to write a short report in the style required by a scientific journal. Module Content: This module will deliver theoretical knowledge of important laboratory techniques in the form of in person lectures and tutorials. Additionally, students will work in teaching laboratories to produce a chimeric antigen receptor (CAR) cell line and measure its effect on the activation of B cells. This will be done in a series of experiments: 1. Transfection of Jurkat cells by nucleofection with a plasmid encoding CD19, 2. Verifying CAR expression on the Jurkats by flow cytometry and 3. Incubation of Jurkat cells with Raji B cells, removing the supernatant and measuring IL-2 with ELISA. Module Assessment: The module will be assessed through a combination of online tests and written assignments. The online test will include multiple choice and short answer questions. The written assignment will include the preparation of a laboratory report in the form of a methods paper. Overall, this module will provide students with a comprehensive understanding of laboratory techniques used in the field of biotherapeutics, and give students the practical know how to produce a CAR cell line and to test its effect. | ||||||||||||||||||||||||||||||||||||||||
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Learning Outcomes 1. To gain a systematic understanding of the theory and practice of the following practical techniques: 1. Animal Cell Culture, 2. Cell Viability Assays, 3. ELISA, 4. Flow cytometry, 5. Transfection and 6. Gene cloning. 2. To gain critical awareness of data analysis and presentation in a professional manner. 3. To acquire specialised tools and knowledge to write a short report in the style required by a scientific journal. | ||||||||||||||||||||||||||||||||||||||||
All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml |
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Indicative Content and
Learning Activities Laboratory Techniques overviewIntroduction to the theory and practice of a number of practical techniques including understanding how to perform basic techniques in molecular biology such as gene cloning, animal cell culture and cell viability assays, transfection, ELISA, and Flow cytometry. Overview of the theory and practice of the biomedical techniques and how they are used to produce biotherapeutics for diseases such as cancer, chronic inflammatory diseases, nervous system or cardiovascular system diseases.LaboratoryHands on practical developing Advanced Therapy Medicinal Products (ATMPs) through 1. Transfection of Jurkat cells by electroporation with a plasmid encoding CD19, 2. Verifying CAR expression on the Jurkats by flow cytometry and 3. Incubation of Jurkat cells with Raji B cells, removing the supernatant and measuring IL-2 with ELISA. | ||||||||||||||||||||||||||||||||||||||||
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Indicative Reading List
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Other Resources None | ||||||||||||||||||||||||||||||||||||||||
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