Module: Introduction to Proteomics & Mass Spectrometry

Latest Module Specifications

Current Academic Year 2025 - 2026

Module Title	Introduction to Proteomics & Mass Spectrometry
Module Code	BTE1027 (ITS: BE532)
Faculty	Biotechnology	School	Science & Health
NFQ level	9	Credit Rating	2.5

Description

The purpose of this module is to introduce participants to the technology currently available to analyse global patterns of gene expression at the protein level. The module will addresss applications of this technology to reveal gene regulation events involved in disease progression as well as potential targets for drug discovery and diagnostics. The module will cover the following aspects of proteomic technologies and research; • Sample preparation from various biological materials (e.g. cell lines, tissue, serum, plasma, etc.) • 2D Gel Electrophoresis, including 2D Difference Gel Electrophoresis (2D DIGE) • Mass Spectrometry (MS) for protein identification and characteristion • Quantitative LC-MS proteomic technologies o SILAC o iTRAQ o Label-free • Introduction to bioinformatics tools for proteomics

Learning Outcomes

1. Select the most appropriate method of sample preparation for various biological materials.
2. 1D761A4D-27FE-0001-61FB-CE9E11F5131D

*Type*	*Hours*	*Description*
Workload	Full time hours per semester
Lecture	12	No Description
Independent Study	3	Reading
Directed learning	1	No Description
Total Workload: 16

Section Breakdown
CRN	20108	Part of Term	Semester 2
Coursework	0%	Examination Weight	0%
Grade Scale		Pass Both Elements	Y
Resit Category		Best Mark	N
Module Co-ordinator	Paula Meleady	Module Teacher	Finbarr O'Sullivan

Type	Description	% of total	Assessment Date
Assessment Breakdown
Short Answer Questions	n/a	100%	Week 1

Reassessment Requirement Type
Resit arrangements are explained by the following categories; RC1: A resit is available for both^* components of the module. RC2: No resit is available for a 100% coursework module. RC3: No resit is available for the coursework component where there is a coursework and summative examination element. ^* ‘Both’ is used in the context of the module having a coursework/summative examination split; where the module is 100% coursework, there will also be a resit of the assessment

Pre-requisite	None
Co-requisite	None
Compatibles	None
Incompatibles	None

All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml

Indicative Content and Learning Activities

Indicative Reading List

Books:
None

Articles:

Gorg et al.: 2004, Current two-dimensional electrophoresis technology for proteomics., Proteomics, 4, 3665-3685, 516126
2003: Mass spectrometry-based proteomics., Nature, 422, 198-207, 516127, 1
Gel-free mass spectrometry-based high throughput proteomics: Tools for studying biological response of proteins and proteomes: Proteomics, 6, 4678-4687, 516128, 1, Zhu et al.
J. Biomed. Biotechnol.: 840518, 516129, 1, Street and Dear, 2010
69(4): 367-378, 516130, 1, Pimienta et al., 2009, SILAC for global phosphoproteomic analysis.
107-116: 516131, 1, Leithner and Lindner, 2009, Chemical strategies for mass spectrometry-based phosphoproteomics., Methods Mol Biol.

Other Resources

None