| Module Title |
Animal Cell Culture Technology |
| Module Code |
BTE1033 (ITS: BE582) |
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Faculty |
Biotechnology |
School |
Science & Health |
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NFQ level |
9 |
Credit Rating |
5 |
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Description
The aim of this module is to give students an understanding of the role of cell culture in bioprocessing engineering. It will outline the basic cellular processes that are of relevance to bioprocessing and provide information about the key criteria to be considered for scale-up of a process.
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Learning Outcomes
1. Gain critical awareness of the different types of cell culture technologies.
2. Gain a systematic understanding of the criteria for consideration for scale up of cell culture.
3. Gain advanced knowledge to identify the appropriate cell model for a large scale process.
4. Gain critical awareness of recent developments in cell and tissue engineering.
5. Gain a systematic understanding to identify key criteria for a successful cell bank.
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| Workload | Full time hours per semester | | Type | Hours | Description |
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| Lecture | 16 | Asynchronous/synchronous/in-person lectures. | | Independent Study | 69 | Reference to reading material | | Assignment Completion | 40 | Complete assignment as directed. | | Lecture | 16 | Asynchronous/synchronous/in-person lectures. | | Independent Study | 69 | Reference to reading material. | | Assignment Completion | 40 | Complete assignment as directed. |
| Total Workload: 250 |
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| Section Breakdown | | CRN | 10173 | Part of Term | Semester 1 | | Coursework | 0% | Examination Weight | 0% | | Grade Scale | 40PASS | Pass Both Elements | Y | | Resit Category | RC1 | Best Mark | N | | Module Co-ordinator | Finbarr O'Sullivan | Module Teacher | Brian Freeland, David Collins, Denise Harold, Janosch Heller, Jonathan Cawley, Paul Cahill |
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| Assessment Breakdown |
| Type | Description | % of total | Assessment Date |
|---|
| Loop Quiz | This loop quiz will consist of a series of problem- or challenge-based MCQs to address the students' critical understanding of the curriculum by MCQs that specifically address the students' knowledge of one specific area and use it in another area; sample questions will be provided to students in advance of the exam. | 40% | Week 10 | | Essay | The students will be asked to critically address a specific question in a concise manner through submission of a short essay (~1500 words). The assignment will be due in week 13. | 60% | Sem 1 End |
| Reassessment Requirement Type |
Resit arrangements are explained by the following categories;
RC1: A resit is available for both* components of the module.
RC2: No resit is available for a 100% coursework module.
RC3: No resit is available for the coursework component where there is a coursework and summative examination element.
* ‘Both’ is used in the context of the module having a coursework/summative examination split; where the module is 100% coursework, there will also be a resit of the assessment
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Pre-requisite |
None
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Co-requisite |
None |
| Compatibles |
None |
| Incompatibles |
None |
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All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml
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Indicative Content and Learning Activities
Basic Cell Biology
Intro to Cell Culture
Types of Tissue culture
Cell Banking
Cell Freezing
Growing Cell - culture conditions
Cell adhesion
Cell cycle and cell proliferation
Cell transformation
Scale-up - batch culture versus continuous culture
Monolayer culture versus suspension culture
3D culture
Stem cells
Proteomic technology
Microarray technology
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Indicative Reading List
Books:
- R. Ian Freshney: 2016, Culture of Animal Cells, 7th Edition, Wiley,
- John R. W. Masters: 2000, Animal Cell Culture, Third Edition, Oxford,
Articles:
- Feng Li, Natarajan Vijayasankaran, Amy Yijuan Shen, Robert Kiss, Ashraf Amanullah: 2010, Cell culture processes for monoclonal antibody production, https://doi.org/10.4161/mabs.2.5.12720, 518163
- 2021: A Review on the Current Methods of Chinese Hamster Ovary (CHO) Cells Cultivation for the Production of Therapeutic Protein, https://doi.org/10.2174/1570163817666200312102137,
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Other Resources
None |
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