| Module Title |
Fundamentals of Molecular Biology |
| Module Code |
GCB1009 (ITS: BE301) |
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Faculty |
Biotechnology |
School |
Science & Health |
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NFQ level |
8 |
Credit Rating |
5 |
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Description
To develop a good understanding of the biology of bacteriophages and plasmids.
To teach the basis of techniques used in the manipulation of DNA/RNA and to provide a theoretical background that would enable students to learn the skills involved in nucleic acid manipulation.
To develop an appreciation and understanding of the complexity of gene expression and its control, with an emphasis on eukaryotic systems.
To give an overview of the content of Eukaryotic genomes and their functionality.
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Learning Outcomes
1. Design and understand recombinant DNA cloning strategies and other relevant molecular biological methodologies useful in the investigation of nucleic acids.
2. Select the appropriate vector system for application in genetic analysis
3. Analyse genome sequences to gain an understanding of gene organisation and function of the encoded information- a focus on the gene itself as a functional unit
4. Design an experimental approach to monitor the expression of genes using RNA analysis
5. Gain knowledge and understanding of the complexity of Eukaryotic Gene Expression control.
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| Workload | Full time hours per semester | | Type | Hours | Description |
|---|
| Tutorial | 12 | No Description | | Lecture | 12 | No Description | | Independent Study | 97 | Reading recommended text books and working through recorded lectures. | | Assignment Completion | 4 | No Description |
| Total Workload: 125 |
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| Section Breakdown | | CRN | 10737 | Part of Term | Semester 1 | | Coursework | 0% | Examination Weight | 0% | | Grade Scale | 40PASS | Pass Both Elements | Y | | Resit Category | RC3 | Best Mark | N | | Module Co-ordinator | Janosch Heller | Module Teacher | Anne Parle-Mcdermott, Denise Harold, Linda Holland, Naomi Walsh, Paul Cahill, Paula Meleady |
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| Assessment Breakdown |
| Type | Description | % of total | Assessment Date |
|---|
| Loop Quiz | 3 Loop quizzes (each worth 3.33%) in weeks 3, 4, 5 covering first half of BE301 | 10% | Other | | Loop Quiz | 3 Loop quizzes (each worth 3.33%) in weeks 9, 10, 11 covering second half of BE301 | 10% | Other | | Formal Examination | Final exam covering BE301. Students will have to answer 4 questions out of a pool of 6, and 2 questions out of a pool of 3. Exam will be 2 hours. | 80% | End-of-Semester |
| Reassessment Requirement Type |
Resit arrangements are explained by the following categories;
RC1: A resit is available for both* components of the module.
RC2: No resit is available for a 100% coursework module.
RC3: No resit is available for the coursework component where there is a coursework and summative examination element.
* ‘Both’ is used in the context of the module having a coursework/summative examination split; where the module is 100% coursework, there will also be a resit of the assessment
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Pre-requisite |
None
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Co-requisite |
None |
| Compatibles |
None |
| Incompatibles |
None |
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All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml
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Indicative Content and Learning Activities
Recombinant DNA technology
- restriction enzymes, joining DNA molecules. DNA amplification, the polymerase chain reaction. Vectors for gene cloningPlasmids, introduction to plasmids
- structure, high and low copy number, different forms, plasmid encoded phenotypes.Replication of plasmids
DNA sequencing methodologies.
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Indicative Reading List
Books:
- Jeremy W. Dale, Simon Park: 0, Molecular genetics of bacteria, 0470850841
- Richard J. Reece: 0, Analysis of genes and genomes, 0470843799
- T. A. Brown: 0, Genetics, 0412447304
- Lewin: 0, GENES XII,
- Strachan and Read: 0, Human Molecular Genetics,
- Mumtaz Anwar, Riyaz Ahmad Rather, Zeenat Farooq: 0, Fundamentals and Advances in Medical Biotechnology,
- Kakoli Bose: 0, Textbook on Cloning, Expression and Purification of Recombinant Proteins,
Articles:
None |
Other Resources
None |
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