| Module Title |
Molecular Cloning and Cell Culture Practicals |
| Module Code |
GCB1018 (ITS: BE380) |
|
Faculty |
Biotechnology |
School |
Science & Health |
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NFQ level |
8 |
Credit Rating |
5 |
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Description
The Practical Molecular and Cell Biology module is designed to provide students with hands-on experience in fundamental molecular and cell biology techniques. The module aims to extend students' understanding of molecular and cell biology concepts, develop key technical laboratory skills, and improve their challenge-based learning by fostering collaboration and critical thinking.
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Learning Outcomes
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| Workload | Full time hours per semester | | Type | Hours | Description |
|---|
| Lecture | 3 | Bioinformatics for Recombinant DNA Analysis | | Online activity | 15 | Bioinformatics assignment preparation | | Laboratory | 24 | Practical experimental work | | Tutorial | 9 | Data analysis and interpretation | | Independent Study | 74 | Preparation before lab, data analysis, literature review, and studying |
| Total Workload: 125 |
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| Section Breakdown | | CRN | 12219 | Part of Term | Semester 1 | | Coursework | 0% | Examination Weight | 0% | | Grade Scale | 40PASS | Pass Both Elements | Y | | Resit Category | RC2 | Best Mark | N | | Module Co-ordinator | Jiafu Wang | Module Teacher | Denise Harold, Emma Finlay, Paul Cahill, Paula Meleady |
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| Assessment Breakdown |
| Type | Description | % of total | Assessment Date |
|---|
| Assignment | Bioinformatics tasks | 30% | n/a | | Report(s) | Laboratory reports | 30% | n/a | | Practical/skills evaluation | Laboratory Proficiency | 10% | n/a | | In Class Test | Include questions on bioinformatics and the recombinant DNA and cell culture practicals | 30% | n/a |
| Reassessment Requirement Type |
Resit arrangements are explained by the following categories;
RC1: A resit is available for both* components of the module.
RC2: No resit is available for a 100% coursework module.
RC3: No resit is available for the coursework component where there is a coursework and summative examination element.
* ‘Both’ is used in the context of the module having a coursework/summative examination split; where the module is 100% coursework, there will also be a resit of the assessment
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Pre-requisite |
None
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Co-requisite |
None |
| Compatibles |
None |
| Incompatibles |
None |
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All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml
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Indicative Content and Learning Activities
Indicative Content
• Recombinant DNA cloning including the following techniques: PCR; gel electrophoresis; DNA purification; restriction digestion; DNA ligation; transformation; clonal selection; plasmid preparation; restriction enzyme mapping
• Expression of target gene in bacterial cells and phenotypic analysis
• Mammalian cell culture and assessment of growth and viability
• Expression of target gene in mammalian cells and microscopy analysis
Practical Manual
GCB1018 Molecular Cloning and Cell Culture Practical Manual
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Indicative Reading List
Books:
None
Articles:
None |
Other Resources
None |
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