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Module Title |
Separation Techniques
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Module Code |
CS302
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School |
School of Chemical Sciences
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Online Module Resources
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Level |
3
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Credit Rating |
5
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Pre-requisite |
None
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Co-requisite |
None
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Module Aims
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To introduce the student to the theory and practices of modern separation techniques.
To provide the necessary background to allow the student to select an appropriate separation technique for a given analysis.
To introduce the student to the application of chromatography in a wide range of environments e.g. industrial, medical, environmental.
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Learning Outcomes
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The student should be able to:
7 Understand the thermodynamic and molecular basis for equilibrium separation.
7 Calculate R', K 5, RS, R, N and H for a given separation.
7 Explain the individual and collective contributions to bond broadening and ways in which they can be minimised.
7 Describe the sample preparation, method development and instrumentation required for Gas Chromatography, Liquid Chromatography and Capillary Electrophoresis.
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Indicative Time Allowances
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Hours
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Lectures |
30
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Tutorials |
6
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Laboratories |
0
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Seminars |
0
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Independent Learning Time |
39
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Total |
75
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Placements |
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Assignments |
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NOTE
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Assume that a 5 credit module load represents approximately 75 hours' work, which includes all teaching, in-course assignments, laboratory work or other specialised training and an estimated private learning time associated with the module.
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Indicative Syllabus
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1. Chemical equilibria; activity, acid-base
titrations,
formation constants, solubility products potentiometric
titrations.
2. Thermodynamics of Separation - distribution coefficients,
chemical potential and conditions for equilibrium.
3. Molecular basis for equilibrium separation - the effect
of molecular structure on the distribution of a compound
between two phases. The empirical approach - Rohrschneider
constant.
4. Chromatography separation - definition and classification
of chromatographic mechanisms, scrption isotherms,
retention behaviour, distribution coefficient, capacity
factor, column selectivity, column efficiency, bond
broadening, diffusion, mass transfer, resolution,
quantitative methods.
5. Gas chromatography - principles, stationary phases and
instrumentation.
6. High performance liquid chromatography - principles
stationary and mobile phases and instrumentation.
7. Capillary electrophoresis - principles, mobile phases and
instrumentation.
8. Sample preparation - solid phase extraction and
derivatisation.
9. Chiral chromatography - stationary and mobile phases for
gas and liquid chromatography.
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| Assessment | | Continuous Assessment | 0% | Examination Weight | 100% |
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Indicative Reading List
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Essential:
Instrumental Methods of Analysis
Willard, Merritt, Dean & Settle - Wadswarlt 1988
Chromatography, 5th edition, fundamentals and techniques. Ed. E.Heftmann.
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Programme or List of Programmes
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| AC | BSc in Chemical and Pharmaceutical Sc. |
| AS | BSc in Analytical Science |
| CF | BSc in Chemistry with French |
| CG | BSc in Chemistry with German |
| SE | BSc Science Education |
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