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Module Title |
Molecular Genetics of Plants and Man
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Module Code |
BE434
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School |
School of Biotechnology
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Online Module Resources
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Level |
4
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Credit Rating |
5
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Pre-requisite |
BE301
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Co-requisite |
None
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Module Aims
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7 Building on the principles of recombinant DNA technology and gene expression introduced in Module BE301, to develop a good understanding of how this knowledge has been extended and developed to analyse and manipulate the genomes of plants and animals, in particular, man.
7 This module aims to provide an understanding of the molecular basis of human 'genetic diseases'.
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Learning Outcomes
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The student should have a clear understanding of:
7 The biology of bacterial-plant interactions and how such interactions can be manipulated to produce genetically altered plants.
7 Plant and animal transformation systems.
7 Development and release of GMO's.
7 The human genome project and its relationship with molecular medicine.
7 The molecular approaches used to map and clone the genes responsible for genetic diseases. The approaches used to test and scan for genetic mutations.
7 The social and ethical issues related to the study and diagnosis of human genetic disorders.
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Indicative Time Allowances
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Hours
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Lectures |
24
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Tutorials |
0
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Laboratories |
0
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Seminars |
0
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Independent Learning Time |
51
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Total |
75
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Placements |
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Assignments |
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NOTE
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Assume that a 5 credit module load represents approximately 75 hours' work, which includes all teaching, in-course assignments, laboratory work or other specialised training and an estimated private learning time associated with the module.
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Indicative Syllabus
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Genetic Analysis and Manipulation of Plants
¿ Agrobacterium and its interaction with plants.
¿ Ti plasmid-based vectors and plant transformation.
¿ Plant transformation with microprojectiles.
¿ Inheritance and expression of chimeric genes in plants.
¿ Insect/viral/herbicide resistant plants.
¿ Transgenic plants for the production of recombinant
products.
¿ GM plants for fuel and food production
¿ GMO release into the environment
Human Genetics
¿ Introduction to genetic diseases: modes of inheritance,
allele/disease frequencies, etc
¿ Human Genome Project, current programme, historical
perspective.
¿ Concept of reverse genetics, genetic and physical markers
(eg, RFLPs, STS, ESTs, SNPs), mapping of genetic disorders,
radiation hybrids, in situ hybridisation, co-inheritance
studies/linkage analysis.
¿ Positional cloning, YACs, linking and jumping libraries,
contigs, PFGE
¿ Some case studies detailing the molecular pathology of
some common human genetic diseases
¿ Methodology used to test for known mutations and to scan
for unknown mutations.
¿ Social and ethical issues raised in the above studies.
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| Assessment | | Continuous Assessment | 0% | Examination Weight | 100% |
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Indicative Reading List
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Essential:
7 Human Molecular Genetics, Strachan and Read (BIOS Scientific, 2000)
7 Human Molecular Genetics, Sudbery (Longman, 1998)
Supplementary:
7 Selection of journal articles.
7 Molecular Basis of Inherited Disease, Davies and Read (In Focus, IRL Press, 1992)
7 Recombinant DNA, Watson, Gilman, Witkowski and Zoller (Scientific American Books, 1992)
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Programme or List of Programmes
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| BT | BSc in Biotechnology |
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