Registry

Module Specifications

Archived Version 2021 - 2022

Module Title	Bioseparations
Module Code	BE516
School	School of Biotechnology
Online Module Resources
Module Co-ordinator	Professor Ian W. Marison	Office Number	X2 09
NFQ level	9	Credit Rating	5
Pre-requisite	None
Co-requisite	None
Compatibles	None
Incompatibles	None

Description

The aim of this module is to provide a detailed understanding of the behaviour of proteins in solution and how their properties may be altered by changing the physical surroundings. To provide an understanding of the theory and practical techniques involved in developing a purification process.

Learning Outcomes

1. Discuss the principles of protein chemistry in solution.
2. Design a DSP process which is readily integrated with the USP
3. Decide how to choose the correct separation and chromatographic techniques.
4. Demonstrate an understanding of the theory and practice of chromatography and how this can help in designing the DSP.
5. Comment on the different types of chromatographic equipment and how they operate

*Type*	*Hours*	*Description*
Workload	Full-time hours per semester
Lecture	16	No Description
Independent Study	109	No Description
Total Workload: 125

All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml

Indicative Content and Learning Activities

Bioseparations
This module is built on the fundamental concepts of protein structure and function, physical chemistry of solutions and electrolytes/ acids/bases and pH to understand how proteins behave in solution and how their properties may be altered by changing the physical surroundings. Using examples, descriptions will be given as to how to isolate proteins from inclusion bodies or culture medium using a range of chemical and physical separation processes. The principles of developing a purification process, including theory, will be defined using a wide range of chromatographic techniques including, but not exclusive to; gel filtration, ion-exchange, hydrophobic interaction, reverse phase, metal chelate, immunoaffinity and other affinity- based techniques, microfiltration and others. The different types of equipment, fluidized versus packed-beds and how these may be scaled-up will be discussed.

Assessment Breakdown
Continuous Assessment	100%	Examination Weight	0%

Type	Description	% of total	Assessment Date
Course Work Breakdown

Reassessment Requirement
Resit arrangements are explained by the following categories; 1 = A resit is available for all components of the module 2 = No resit is available for 100% continuous assessment module 3 = No resit is available for the continuous assessment component
Unavailable

Indicative Reading List

Robert K.: 1994, Protein Purification – Principles & Practice, 3rd Edition, Scopes/Springer Verlag,
Paul Belter, E.L. Cussler, Wei-Shou Hu: 1988, Bioseperation Downstream Processing for Biotechnology, John Wiley & Sons,

Other Resources

None

Programme or List of Programmes

AMPD	PhD
AMPM	MSc
AMPT	PhD-track
BTPD	PhD
BTPM	MSc
BTPT	PhD-track
CHPD	PhD
CHPM	Master of Science
CHPT	PhD-track
GCBE	Graduate Cert in Bioprocess Engineering
GDBE	Graduate Dip in Bioprocess Engineering
GTSH	Graduate Training Visitor Program (S&H)
HHPD	PhD
MSBE	MSc in Bioprocess Engineering
NSPD	PhD
NSPM	MSc
NSPT	PhD-track
PHPD	PhD
PHPM	MSc
PHPT	PhD-track
PYPD	PhD
PYPM	MSc
PYPT	PhD-track
SMPSC	Single Module Prof. Science and Health
SSPD	PhD
SSPM	MSc
SSPT	PhD-track

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