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Module Specifications

Archived Version 2003 - 2004

Module Title Bioanalytical Practicals 1
Module Code BE353
School School of Biotechnology

Online Module Resources

Level 3 Credit Rating 5
Pre-requisite None
Co-requisite None
Module Aims
This module aims: o To provide students with a good practical 'hands-on' experience of a sequential multistep protein purification scheme. o To provde a detailed understanding of the methods used in practical immumology. o To train students in practical analytical microbiology, particularly in regard to analysis of food and water samples and to introduce basic methods in microbial fermentation.

Learning Outcomes
Students will attain: o Practical experience of a sequential 'multistep' protein purification scheme. o Working knowledge of ion-exchange and gel filtration chromatography. o Practical experience of the isolation and analysis of antibodies. o Introduction to some basic antibody-based analytical methods. o Basic practical experience of HPLC analysis. o Technical ability to carry out batch and continuous microbial culture. o Ability to undertake microbial analsyis of food and water samples.

Indicative Time Allowances
Lectures 0
Tutorials 3
Laboratories 72
Seminars 0
Independent Learning Time 0

Total 75
Assume that a 5 credit module load represents approximately 75 hours' work, which includes all teaching, in-course assignments, laboratory work or other specialised training and an estimated private learning time associated with the module.

Indicative Syllabus
o Assay for lyzozyme activity and protein o Preparation of crude lyzozyme from egg-white o Salt precipitation of lyzozyme by gel-filtration chromatography on sephadex G-100 o Purification of 'partially-purified' lyzozyme by cation-exchange chromatography o Concentration of lyzozyme by reverse dialysis using solid polyethylene glycol o Analysis of lyzozyme fractions by native and SDS Polyacrylamide electrophoresis o Analysis of lyzozyme fractions and standards by Isoelectric focusing o Isolation of antibodies - salt precipitation,estimation of recoveries and yields o Isoelectric focusing- analysis of standards,serum and antibody fractions o Immunoelectrophoresis - analysis of standards,serum and antibody fractions o Measurement of antibody levels by enzyme-linked immunosorbent assay (ELISA) o Computer learning system for teaching the use of HPLC and its applications o Techniques to prepare and analyse samples for the HPLC analysis of drugs o Determination of molar growth yield in batch and continuous microbial fermentation o Food microbiology - analysis of samples by pour plates and spread plates o Water microbiology - analysis of samples by MPN and membrane filtration
Continuous Assessment100% Examination Weight0%
Indicative Reading List
Essential: o 'Protein purification and analysis' 3rd year practical manual o 'Microbiology' 3rd year practical manual o 'Immunology' 3rd year practical manual Supplementary: o 'Protein purification methods'by Harris and Angal (Oxford,1990) o 'Practical Immunology' by Hudson and Hay (Blackwell,1994) o 'Laboratory Methods in Food and Dairy Microbiology' by Harigan & McCrance o 'Sourcebook of Experiments for the Teaching of Microbiology' by Primrose and Wordlaw
Programme or List of Programmes
ASBSc in Analytical Science