Registry
Module Specifications
Archived Version 2003 - 2004
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Module Title |
Bioanalytical Practicals 1
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Module Code |
BE353
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School |
School of Biotechnology
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Online Module Resources
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Level |
3
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Credit Rating |
5
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Pre-requisite |
None
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Co-requisite |
None
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Module Aims
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This module aims:
o To provide students with a good practical 'hands-on' experience of a sequential multistep protein purification scheme.
o To provde a detailed understanding of the methods used in practical immumology.
o To train students in practical analytical microbiology, particularly in regard to analysis of food and water samples and to introduce basic methods in microbial fermentation.
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Learning Outcomes
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Students will attain:
o Practical experience of a sequential 'multistep' protein purification scheme.
o Working knowledge of ion-exchange and gel filtration chromatography.
o Practical experience of the isolation and analysis of antibodies.
o Introduction to some basic antibody-based analytical methods.
o Basic practical experience of HPLC analysis.
o Technical ability to carry out batch and continuous microbial culture.
o Ability to undertake microbial analsyis of food and water samples.
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Indicative Time Allowances
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Hours
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Lectures |
0
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Tutorials |
3
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Laboratories |
72
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Seminars |
0
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Independent Learning Time |
0
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Total |
75
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Placements |
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Assignments |
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NOTE
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Assume that a 5 credit module load represents approximately 75 hours' work, which includes all teaching, in-course assignments, laboratory work or other specialised training and an estimated private learning time associated with the module.
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Indicative Syllabus
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o Assay for lyzozyme activity and protein
o Preparation of crude lyzozyme from egg-white
o Salt precipitation of lyzozyme by gel-filtration chromatography on sephadex G-100
o Purification of 'partially-purified' lyzozyme by cation-exchange chromatography
o Concentration of lyzozyme by reverse dialysis using solid polyethylene glycol
o Analysis of lyzozyme fractions by native and SDS Polyacrylamide electrophoresis
o Analysis of lyzozyme fractions and standards by Isoelectric focusing
o Isolation of antibodies - salt precipitation,estimation of recoveries and yields
o Isoelectric focusing- analysis of standards,serum and antibody fractions
o Immunoelectrophoresis - analysis of standards,serum and antibody fractions
o Measurement of antibody levels by enzyme-linked immunosorbent assay (ELISA)
o Computer learning system for teaching the use of HPLC and its applications
o Techniques to prepare and analyse samples for the HPLC analysis of drugs
o Determination of molar growth yield in batch and continuous microbial fermentation
o Food microbiology - analysis of samples by pour plates and spread plates
o Water microbiology - analysis of samples by MPN and membrane filtration
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| Assessment | | Continuous Assessment | 100% | Examination Weight | 0% |
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Indicative Reading List
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Essential:
o 'Protein purification and analysis' 3rd year practical manual
o 'Microbiology' 3rd year practical manual
o 'Immunology' 3rd year practical manual
Supplementary:
o 'Protein purification methods'by Harris and Angal (Oxford,1990)
o 'Practical Immunology' by Hudson and Hay (Blackwell,1994)
o 'Laboratory Methods in Food and Dairy Microbiology' by Harigan & McCrance
o 'Sourcebook of Experiments for the Teaching of Microbiology' by Primrose and Wordlaw
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Programme or List of Programmes
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| AS | BSc in Analytical Science |
| Archives: | |
