Registry
Module Specifications
Archived Version 2009 - 2010
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Module Title |
Bioanalytical Practicals I
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Module Code |
BE353
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School |
School of Biotechnology
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Online Module Resources
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Level |
3
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Credit Rating |
5
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Pre-requisite |
None
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Co-requisite |
None
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Module Aims
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This module aims:·
- To provide students with a good practical 'hands-on' experience of a sequential multistep protein purification scheme.·
- To provde a detailed understanding of the methods used in practical immumology.·
- To train students in practical analytical microbiology, particularly in regard to analysis of food and water samples and to introduce basic methods in microbial fermentation.
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Learning Outcomes
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Students will attain:·
- Practical experience of a sequential 'multistep' protein purification scheme.
- Working knowledge of ion-exchange and gel filtration chromatography.
- Practical experience of the isolation and analysis of antibodies.
- Introduction to some basic antibody-based analytical methods.
- Basic practical experience of HPLC analysis.
- Technical ability to carry out batch and continuous microbial culture.
- Ability to undertake microbial analsyis of food and water samples.
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Indicative Time Allowances
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Hours
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Lectures |
0
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Tutorials |
3
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Laboratories |
72
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Seminars |
0
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Independent Learning Time |
0
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Total |
75
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Placements |
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Assignments |
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NOTE
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Assume that a 5 credit module load represents approximately 75 hours' work, which includes all teaching, in-course assignments, laboratory work or other specialised training and an estimated private learning time associated with the module.
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Indicative Syllabus
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- Assay for lyzozyme activity and protein·
- Preparation of crude lyzozyme from egg-white·
- Salt precipitation of lyzozyme by gel-filtration chromatography on sephadex G-100 ·Purification of ''''''''partially-purified'''''''' lyzozyme by cation-exchange chromatography·
- Concentration of lyzozyme by reverse dialysis using solid polyethylene glycol·
- Analysis of lyzozyme fractions by native and SDS Polyacrylamide electrophoresis·
- Analysis of lyzozyme fractions and standards by Isoelectric focusing·Isolation of antibodies - salt precipitation,estimation of recoveries and yields·
- Isoelectric focusing- analysis of standards,serum and antibody fractions·Immunoelectrophoresis - analysis of standards,serum and antibody fractions·
- Measurement of antibody levels by enzyme-linked immunosorbent assay (ELISA)·
- Computer learning system for teaching the use of HPLC and its applications·
- Techniques to prepare and analyse samples for the HPLC analysis of drugs·Determination of molar growth yield in batch and continuous microbial fermentation·
- Food microbiology - analysis of samples by pour plates and spread plates·Water microbiology - analysis of samples by MPN and membrane filtration
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| Assessment | | Continuous Assessment | 100% | Examination Weight | 0% |
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Indicative Reading List
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Essential: ·
'Protein purification and analysis' 3rd year practical manual·'Microbiology' 3rd year practical manual·'Immunology' 3rd year practical manualSupplementary: ·'Protein purification methods'by Harris and Angal (Oxford,1990)·'Practical Immunology' by Hudson and Hay (Blackwell,1994)·'Laboratory Methods in Food and Dairy Microbiology' by Harigan & McCrance·'Sourcebook of Experiments for the Teaching of Microbiology' by Primrose and Wordlaw
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Programme or List of Programmes
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| AS | BSc in Analytical Science |
| Archives: | |
