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Module Specifications

Archived Version 2010 - 2011

Module Title Bioanalytical Practicals I
Module Code BE353
School School of Biotechnology

Online Module Resources

Level 3 Credit Rating 5
Pre-requisite None
Co-requisite None
Module Aims

This module aims:·

  • To provide students with a good practical 'hands-on' experience of a sequential multistep protein purification scheme.·
  • To provde a detailed understanding of the methods used in practical immumology.·
  • To train students in practical analytical microbiology, particularly in regard to analysis of food and water samples and to introduce basic methods in microbial fermentation.

Learning Outcomes

Students will attain:·

  • Practical experience of a sequential 'multistep' protein purification scheme.
  • Working knowledge of ion-exchange and gel filtration chromatography.
  • Practical experience of the isolation and analysis of antibodies.
  • Introduction to some basic antibody-based analytical methods.
  • Basic practical experience of HPLC analysis.
  • Technical ability to carry out batch and continuous microbial culture.
  • Ability to undertake microbial analsyis of food and water samples.

Indicative Time Allowances
Lectures 0
Tutorials 3
Laboratories 72
Seminars 0
Independent Learning Time 0

Total 75
Assume that a 5 credit module load represents approximately 75 hours' work, which includes all teaching, in-course assignments, laboratory work or other specialised training and an estimated private learning time associated with the module.

Indicative Syllabus
  • Assay for lyzozyme activity and protein·
  • Preparation of crude lyzozyme from egg-white·
  • Salt precipitation of lyzozyme by gel-filtration chromatography on sephadex G-100 ·Purification of ''''''''partially-purified'''''''' lyzozyme by cation-exchange chromatography·
  • Concentration of lyzozyme by reverse dialysis using solid polyethylene glycol·
  • Analysis of lyzozyme fractions by native and SDS Polyacrylamide electrophoresis·
  • Analysis of lyzozyme fractions and standards by Isoelectric focusing·Isolation of antibodies - salt precipitation,estimation of recoveries and yields·
  • Isoelectric focusing- analysis of standards,serum and antibody fractions·Immunoelectrophoresis - analysis of standards,serum and antibody fractions·
  • Measurement of antibody levels by enzyme-linked immunosorbent assay (ELISA)·
  • Computer learning system for teaching the use of HPLC and its applications·
  • Techniques to prepare and analyse samples for the HPLC analysis of drugs·Determination of molar growth yield in batch and continuous microbial fermentation·
  • Food microbiology - analysis of samples by pour plates and spread plates·Water microbiology - analysis of samples by MPN and membrane filtration
Continuous Assessment100% Examination Weight0%
Indicative Reading List

Essential: ·

'Protein purification and analysis' 3rd year practical manual·'Microbiology' 3rd year practical manual·'Immunology' 3rd year practical manualSupplementary: ·'Protein purification methods'by Harris and Angal (Oxford,1990)·'Practical Immunology' by Hudson and Hay (Blackwell,1994)·'Laboratory Methods in Food and Dairy Microbiology' by Harigan & McCrance·'Sourcebook of Experiments for the Teaching of Microbiology' by Primrose and Wordlaw

Programme or List of Programmes
ASBSc in Analytical Science