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Module Aims
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- To review the use of enzymes in key industrial processes, both traditional and recently-evolved, with emphasis on their underlying biotechnological strengths and current limitations.
- To outline how biotechnological innovations (especially protein engineering) have resulted in improved products and processes.
- To provide an overview of biosensor development.
- To emphasize the importance of purification strategies and downstream processing in the large scale production and recovery of commercial biotechnology products.
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Learning Outcomes
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Students will gain:
- An overview of the uses and advantages of enzymes in starch hydrolysis, antibiotic modification and other industrial processes
- An overview of structure
-determination and bioinformatics techniques used in protein engineering
- An understanding of the potential of protein engineering for the tailoring of useful proteins
- An appreciation of the scope and potential of enzyme and biosensor technologies in the evolution of current, developing and innovative products/processes- An understanding of the basis of a variety of protein separation methodologies
- A grounding in modern chromatographic methods and their applications
- The ability to discuss/ predict possible future developments in specific biotechnological industries in the context of developing technologies.
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Indicative Time Allowances
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Hours
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Lectures |
30
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Tutorials |
2
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Laboratories |
0
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Seminars |
3
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Independent Learning Time |
40
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Total |
75
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Placements |
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Assignments |
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NOTE
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Assume that a 5 credit module load represents approximately 75 hours' work, which includes all teaching, in-course assignments, laboratory work or other specialised training and an estimated private learning time associated with the module.
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Indicative Syllabus
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INDUSTRIAL ENZYMOLOGY
Sources and production of industrial enzymes; enzymes in detergents;
starch processing and high-fructose syrup;
use of enzymes for antibiotic semisynthesis; leather processing;
cellulose degradation in the textile and paper industries.
PROTEIN ENGINEERING
Underlying technologies, incl. databases and bioinformatics;
Case history - subtilisin; Insulin and its semisynthesis; Directed evolution of an enzyme.
BIOSENSORS
Definition & applications; biocomponents; transducers; design problems; electrochemical sensors incl. examples; luminescence; surface plasmon resonance; scanning probe microscopy.
DOWNSTREAM PROCESSING AND PRODUCT RECOVERY:
Advanced separations (affinity, immuno-, metal chelate, covalent, lectin, heparin-SepharoseTM and triazine dye chromatographies, chromatofocusing); Equipment for chromatography (HPLC, MPLC, FPLC, BioPilot); Large scale purifications and problems of scale-up: process design and optimization, primary and batch operations; Examples; Protein engineering for purification.
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| Assessment | | Continuous Assessment | 0% | Examination Weight | 100% |
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Indicative Reading List
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Essential:
Proteins: Biochemistry & Biotechnology. G Walsh. J Wiley & Sons 2002
Enzyme Technology M.F. Chaplin & C. Bucke Cambridge University Press 1990
Industrial Enzymology 2nd ed. T. Godfrey & S. West Macmillan 1996
Protein Purification Methods Harris, E.L.V. & Angal, S. IRL Press 1989
Protein Purification Applications Harris, E.L.V. & Angal, S. IRL Press 1990
Protein Engineering Moody, P.C.E. & Wilkinson, A.J. IRL Press 1990
Supplementary:
Proteins (2nd edn) T.E. Creighton Freeman 1993
Resource and applications of biotechnology R. Greenshields
Microbial Proteinases H.M Kalisz in Advances in Biochemical Engineering vol 36 pp 1-65
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Programme or List of Programmes
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| BT | BSc in Biotechnology |
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