DCU Home | Our Courses | Loop | Registry | Library | Search DCU


Module Specifications

Archived Version 2007 - 2008

Module Title Proteomics and Protein Biotechnology
Module Code BE402
School School of Biotechnology

Online Module Resources

Module Co-ordinatorDr Ciaran FaganOffice NumberX207
Level 4 Credit Rating 5
Pre-requisite None
Co-requisite None
Module Aims

Module Aims:-

  • To review the use of enzymes in key industrial processes, both traditional and recently-evolved, with emphasis on their underlying biotechnological strengths and current limitations
  • To outline how biotechnological innovations (especially protein engineering) have resulted in improved products and processes
  • To provide an overview of biosensor development
  • To understand the relevance of DNA recombinant technology for the production & purification of proteins and peptides
  • To understand key techniques used for the purification and analysis of peptides & proteins
  • To provide an overview of proteomics
  • To review problems associated with large scale/scale-up production of proteins

Learning Outcomes

Students will gain:-

  • An overview of the uses and advantages of enzymes in starch hydrolysis, antibiotic modification and other industrial processes
  • An overview of techniques and resources underpinning protein engineering
  • An understanding of the potential of protein engineering for the tailoring of useful proteins
  • An appreciation of the scope and potential of enzyme and biosensor technologies in the evolution of current, developing and innovative products/processes
  • An understanding of the basis of a variety of modern protein separation/analytical methodologies
  • An appreciation of the applications of DNA recombinant technology to the production of proteins.
  • An overview of Proteomics

Indicative Time Allowances
Lectures 30
Tutorials 2
Laboratories 0
Seminars 3
Independent Learning Time 40

Total 75
Assume that a 5 credit module load represents approximately 75 hours' work, which includes all teaching, in-course assignments, laboratory work or other specialised training and an estimated private learning time associated with the module.

Indicative Syllabus

Indicative Syllabus:


Sources and production of industrial enzymes; enzymes in detergents; starch processing and high-fructose syrup; use of enzymes for antibiotic semisynthesis; leather processing; cellulose degradation in the textile and paper industries.


Underlying technologies, incl. databases and bioinformatics; Case history - subtilisin; Insulin and its semisynthesis; Directed evolution of an enzyme.BIOSENSORSDefinition & applications; biocomponents; transducers; design problems; electrochemical sensors incl. examples; luminescence; surface plasmon resonance; scanning probe microscopy.


Purification of Recombinant proteins (His-tag & Thioredoxin fusions , inclusion body formation etc).Genetic Manipulation of protein source for optimal protein/peptide production and stability.Advanced Separations (Affinity, Metal-chelate (IMAC), Covalent Chromatographies Capilliary Electrophoresis - Protein & Peptide Analysis.Large scale protein purification and associated problems of scale-up.Introduction to the basic concepts of Proteomics Appplications of Proteomics

Continuous Assessment0% Examination Weight100%
Indicative Reading List

Indicative Reading List:


Protein Biochemistry & Biotechnology G.K. Walsh Wiley (2002) .Cloning, Gene Expression and Protein Purification Hardin, C et al. Oxford University Press (2001).Protein Purification Bonner , P. Taylor & Francis (2006)Protein Purification Techniques Roe, Simon. Oxford Press (2001)Protein Engineering Moody, P.C.E. & Wilkinson, A.J. IRL Press (1990)


Bound BE402 reference papers: Library Restricted Loan Collection.Protein Purification Methods Harris, E.L.V. & Angal, S. IRL Press (1995)Protein Biotechnology G.K. Walsh & D.G. Headon Wiley (1996)Resource and applications of biotechnology R. Greenshields (2003)Microbial Proteinases H.M Kalisz in Advances in Biochemical Engineering vol 36 pp 1-65

Programme or List of Programmes
BSSAStudy Abroad (DCU Business School)
BSSAOStudy Abroad (DCU Business School)
BTBSc in Biotechnology
ECSAStudy Abroad (Engineering & Computing)
ECSAOStudy Abroad (Engineering & Computing)
GCBBSc in Genetics & Cell Biology
HMSAStudy Abroad (Humanities & Soc Science)
HMSAOStudy Abroad (Humanities & Soc Science)
SHSAStudy Abroad (Science & Health)
SHSAOStudy Abroad (Science & Health)