Registry

Module Specifications

Archived Version 2007 - 2008

Module Title	Proteomics and Protein Biotechnology
Module Code	BE402
School	School of Biotechnology
Online Module Resources
Module Co-ordinator	Dr Ciaran Fagan	Office Number	X207
Level	4	Credit Rating	5
Pre-requisite	None
Co-requisite	None

Module Aims

Module Aims:-

To review the use of enzymes in key industrial processes, both traditional and recently-evolved, with emphasis on their underlying biotechnological strengths and current limitations
To outline how biotechnological innovations (especially protein engineering) have resulted in improved products and processes
To provide an overview of biosensor development
To understand the relevance of DNA recombinant technology for the production & purification of proteins and peptides
To understand key techniques used for the purification and analysis of peptides & proteins
To provide an overview of proteomics
To review problems associated with large scale/scale-up production of proteins

Learning Outcomes

Students will gain:-

An overview of the uses and advantages of enzymes in starch hydrolysis, antibiotic modification and other industrial processes
An overview of techniques and resources underpinning protein engineering
An understanding of the potential of protein engineering for the tailoring of useful proteins
An appreciation of the scope and potential of enzyme and biosensor technologies in the evolution of current, developing and innovative products/processes
An understanding of the basis of a variety of modern protein separation/analytical methodologies
An appreciation of the applications of DNA recombinant technology to the production of proteins.
An overview of Proteomics

Indicative Time Allowances
	Hours
Lectures	30
Tutorials	2
Laboratories	0
Seminars	3
Independent Learning Time	40

Total	75
Placements
Assignments

NOTE

Assume that a 5 credit module load represents approximately 75 hours' work, which includes all teaching, in-course assignments, laboratory work or other specialised training and an estimated private learning time associated with the module.

Indicative Syllabus

Indicative Syllabus:

INDUSTRIAL ENZYMOLOGY

Sources and production of industrial enzymes; enzymes in detergents; starch processing and high-fructose syrup; use of enzymes for antibiotic semisynthesis; leather processing; cellulose degradation in the textile and paper industries.

PROTEIN ENGINEERING

Underlying technologies, incl. databases and bioinformatics; Case history - subtilisin; Insulin and its semisynthesis; Directed evolution of an enzyme.BIOSENSORSDefinition & applications; biocomponents; transducers; design problems; electrochemical sensors incl. examples; luminescence; surface plasmon resonance; scanning probe microscopy.

PROTEIN PURIFICATION & ANALYSIS

Purification of Recombinant proteins (His-tag & Thioredoxin fusions , inclusion body formation etc).Genetic Manipulation of protein source for optimal protein/peptide production and stability.Advanced Separations (Affinity, Metal-chelate (IMAC), Covalent Chromatographies Capilliary Electrophoresis - Protein & Peptide Analysis.Large scale protein purification and associated problems of scale-up.Introduction to the basic concepts of Proteomics Appplications of Proteomics

Assessment
Continuous Assessment	0%	Examination Weight	100%

Indicative Reading List

Indicative Reading List:

Essential:

Protein Biochemistry & Biotechnology G.K. Walsh Wiley (2002) .Cloning, Gene Expression and Protein Purification Hardin, C et al. Oxford University Press (2001).Protein Purification Bonner , P. Taylor & Francis (2006)Protein Purification Techniques Roe, Simon. Oxford Press (2001)Protein Engineering Moody, P.C.E. & Wilkinson, A.J. IRL Press (1990)

Supplementary:

Bound BE402 reference papers: Library Restricted Loan Collection.Protein Purification Methods Harris, E.L.V. & Angal, S. IRL Press (1995)Protein Biotechnology G.K. Walsh & D.G. Headon Wiley (1996)Resource and applications of biotechnology R. Greenshields (2003)Microbial Proteinases H.M Kalisz in Advances in Biochemical Engineering vol 36 pp 1-65

Programme or List of Programmes

BSSA

Study Abroad (DCU Business School)

BSSAO

Study Abroad (DCU Business School)

BSc in Biotechnology

ECSA

Study Abroad (Engineering & Computing)

ECSAO

Study Abroad (Engineering & Computing)

GCB

BSc in Genetics & Cell Biology