DCU Home | Our Courses | Loop | Registry | Library | Search DCU


Module Specifications

Archived Version 2004 - 2005

Module Title Protein Biotechnology
Module Code BE402
School School of Biotechnology

Online Module Resources

Module Co-ordinatorDr Ciaran FaganOffice NumberX207
Level 4 Credit Rating 5
Pre-requisite BE301, BE302, BE351, BE352
Co-requisite None
Module Aims
- To review the use of enzymes in key industrial processes, both traditional and recently-evolved, with emphasis on their underlying biotechnological strengths and current limitations. - To outline how biotechnological innovations (especially protein engineering) have resulted in improved products and processes. - To provide an overview of biosensor development. - To emphasize the importance of purification strategies and downstream processing in the large scale production and recovery of commercial biotechnology products.

Learning Outcomes
Students will gain: - An overview of the uses and advantages of enzymes in starch hydrolysis, antibiotic modification and other industrial processes - An overview of structure -determination and bioinformatics techniques used in protein engineering - An understanding of the potential of protein engineering for the tailoring of useful proteins - An appreciation of the scope and potential of enzyme and biosensor technologies in the evolution of current, developing and innovative products/processes- An understanding of the basis of a variety of protein separation methodologies - A grounding in modern chromatographic methods and their applications - The ability to discuss/ predict possible future developments in specific biotechnological industries in the context of developing technologies.

Indicative Time Allowances
Lectures 30
Tutorials 2
Laboratories 0
Seminars 3
Independent Learning Time 40

Total 75
Assume that a 5 credit module load represents approximately 75 hours' work, which includes all teaching, in-course assignments, laboratory work or other specialised training and an estimated private learning time associated with the module.

Indicative Syllabus
INDUSTRIAL ENZYMOLOGY Sources and production of industrial enzymes; enzymes in detergents; starch processing and high-fructose syrup; use of enzymes for antibiotic semisynthesis; leather processing; cellulose degradation in the textile and paper industries. PROTEIN ENGINEERING Underlying technologies, incl. databases and bioinformatics; Case history - subtilisin; Insulin and its semisynthesis; Directed evolution of an enzyme. BIOSENSORS Definition & applications; biocomponents; transducers; design problems; electrochemical sensors incl. examples; luminescence; surface plasmon resonance; scanning probe microscopy. DOWNSTREAM PROCESSING AND PRODUCT RECOVERY: Advanced separations (affinity, immuno-, metal chelate, covalent, lectin, heparin-SepharoseTM and triazine dye chromatographies, chromatofocusing); Equipment for chromatography (HPLC, MPLC, FPLC, BioPilot); Large scale purifications and problems of scale-up: process design and optimization, primary and batch operations; Examples; Protein engineering for purification.
Continuous Assessment0% Examination Weight100%
Indicative Reading List
Essential: Proteins: Biochemistry & Biotechnology. G Walsh. J Wiley & Sons 2002 Enzyme Technology M.F. Chaplin & C. Bucke Cambridge University Press 1990 Industrial Enzymology 2nd ed. T. Godfrey & S. West Macmillan 1996 Protein Purification Methods Harris, E.L.V. & Angal, S. IRL Press 1989 Protein Purification Applications Harris, E.L.V. & Angal, S. IRL Press 1990 Protein Engineering Moody, P.C.E. & Wilkinson, A.J. IRL Press 1990 Supplementary: Proteins (2nd edn) T.E. Creighton Freeman 1993 Resource and applications of biotechnology R. Greenshields Microbial Proteinases H.M Kalisz in Advances in Biochemical Engineering vol 36 pp 1-65
Programme or List of Programmes
BTBSc in Biotechnology