Registry

Module Specifications

Archived Version 2012 - 2013

Module Title	ProteOmics and Protein Biotechnology
Module Code	BE402
School	School of Biotechnology
Online Module Resources
Module Co-ordinator	Dr Ciaran Fagan	Office Number	X207
NFQ level	8	Credit Rating	5
Pre-requisite	None
Co-requisite	None
Compatibles	None
Incompatibles	None

Description

To review the use of enzymes in key industrial processes, both traditional and recently-evolved, with emphasis on their underlying biotechnological strengths and current limitations. To outline how biotechnological innovations (especially protein engineering) have resulted in improved products and processes. To provide an overview of biosensor development. To illustrate the relevance of DNA recombinant technology for the production and purification of proteins and peptides. To describe some important techniques used for the purification and analysis of peptides and proteins. To review problems associated with large scale/scale-up production of proteins.

Learning Outcomes

1. Discuss the uses and advantages of bulk enzymes in key industrial processes (detergents, starch processing, antibiotics and cellulose degradation)
2. Describe the basis of a variety of contemporary protein separation/analytical methodologies
3. Provide an overview of techniques and resources underpinning protein engineering
4. Illustrate, using examples, the potential of protein engineering for the tailoring of useful proteins
5. Outline the scope and potential of enzyme and biosensor technologies in the evolution of current, developing and innovative products/processes
6. Outline the applications of DNA recombinant technology to the production of proteins
7. Provide an overview of proteomics

*Type*	*Hours*	*Description*
Workload	Full-time hours per semester
Lecture	36	No Description
Independent Study	89	No Description
Total Workload: 125

All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml

Indicative Content and Learning Activities

INDUSTRIAL ENZYMOLOGY
Sources and production of industrial enzymes; enzymes in detergents; starch processing and high-fructose syrup; use of enzymes for antibiotic semisynthesis; leather processing; cellulose degradation in the textile and paper industries.

PROTEIN ENGINEERING
Underlying technologies, incl. databases and bioinformatics; Case history - subtilisin; Insulin and its semisynthesis; Directed evolution of an enzyme.

BIOSENSORS
Definition & applications; biocomponents; transducers; design problems; developments in biosensors incl. examples.

PROTEIN PURIFICATION & ANALYSIS
Advanced Separations (Affinity, Metal-chelate (IMAC), Covalent Chromatographies ); Purification of Recombinant proteins (His-tag & Thioredoxin fusions, inclusion body formation etc); Genetic Manipulation of protein source for optimal protein/peptide production and stability;Capillary Electrophoresis - Protein & Peptide Analysis; Large scale protein purification and associated problems of scale-up;Introduction to the concepts of Proteomics and Protein Microarrays

BIOPHARMA & RECOMBINANT PROTEINS
Introduction to Biopharma; Protein drug delivery systems

Assessment Breakdown
Continuous Assessment	0%	Examination Weight	100%

Type	Description	% of total	Assessment Date
Course Work Breakdown

Reassessment Requirement
Resit arrangements are explained by the following categories; 1 = A resit is available for all components of the module 2 = No resit is available for 100% continuous assessment module 3 = No resit is available for the continuous assessment component
Unavailable

Indicative Reading List

GK Walsh: 2002, Protein Biochemistry & Biotechnology, Wiley, 978471899075
C Hardin et al.: 2001, Cloning, Gene Expression and Protein Purification, Oxford Univ Press,
P Bonner: 2006, Protein Purification, Taylor & Francis,
S Roe: 2001, Protein Purification Techniques, Oxford Univ Press,
MF Chaplin & C Bucke: 1990, Enzyme Technology, Cambridge Univ Press,
PCE Moody & AJ Wilkinson: 1990, Protein Engineering, IRL Press, 9780199631940
ELV Harris & S Angal: 1995, Protein Purification Methods, IRL Press,
ELV Harris & S Angal: 1994, Protein Purification Applications, IRL Press,
R Greenshields: 2003, Resource and applications of biotechnology,
HM Kalisz: 0, Advances in Biochemical Engineering vol 36, Microbial Proteinases,

Other Resources

None

Programme or List of Programmes

BSSA	Study Abroad (DCU Business School)
BSSAO	Study Abroad (DCU Business School)
BT	BSc in Biotechnology
ECSA	Study Abroad (Engineering & Computing)
ECSAO	Study Abroad (Engineering & Computing)
GCB	BSc in Genetics & Cell Biology
HMSA	Study Abroad (Humanities & Soc Science)
HMSAO	Study Abroad (Humanities & Soc Science)
SHSA	Study Abroad (Science & Health)
SHSAO	Study Abroad (Science & Health)

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