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Module Specifications

Archived Version 2022 - 2023

Module Title Proteins, Proteomics & Biopharma
Module Code BE402
School School of Biotechnology

Online Module Resources

Module Co-ordinatorDr Ciaran FaganOffice NumberX207
NFQ level 8 Credit Rating 5
Pre-requisite None
Co-requisite None
Compatibles None
Incompatibles None

To describe some important techniques used for the purification and analysis of proteins. To illustrate the relevance of DNA recombinant technology to the production and purification of proteins and peptides. To review the use of enzymes in key industrial processes, noting their strengths and limitations. To show how protein engineering has led to improved biotherapeutic products and processes. To provide an overview of biosensor development. To review recent developments in biopharmacology and next generation biotherapeutic modalities.

Learning Outcomes

1. Discuss the applications of bulk enzymes in key industrial processes (e.g. detergents, starch processing).
2. Describe examples of the improvement of key proteins by protein engineering strategies.
3. Outline the scope and potential of biosensor technologies.
4. Outline the applications of DNA recombinant technology to the production and purification of proteins and monoclonal antibodies.
5. Provide an overview of proteomics and protein mass spectrometry analysis.
6. To describe developments in biopharmacology and next generation biotherapeutic modalities.

Workload Full-time hours per semester
Type Hours Description
Lecture24No Description
Online activity12.5Loop MCQ(s )
Assignment Completion5Short Essay on assigned topic
Assignment Completion7.5Critical essay on assigned topic
Independent Study76No Description
Total Workload: 125

All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml

Indicative Content and Learning Activities

Sources and production of industrial enzymes; enzymes in detergents; starch processing and high-fructose syrup; use of enzymes for antibiotic semisynthesis and related purposes; enzyme uses in cellulose treatment and other situations.

Case histories –. E.g. Erythropoietin (EPO); Insulin production and improvement via protein engineering.

Definition & applications; design problems; developments in biosensors incl. examples.

The use of recombinant DNA technology for optimal protein production. Affinity purification of recombinant fusion proteins (His-tag & Thioredoxin fusions) and monoclonal antibodies therapeutics (e.g. Protein A/G, cation or anion exchange chromatography, etc.)

An overview of proteomic analysis, practical methodologies and applications, e.g. 2D-PAGE and LC-MS for protein identification and characterisation (e.g. post translational modifications)

Introduction to Biopharmacology and the importance of protein glycosylation. Overview of some important biotherapeutics for disease treatment. Overview of next generation biotherapeutic modalities (e.g. bi-specific antibodies, antibody drug conjugates, nanobodies, etc.).

Assessment Breakdown
Continuous Assessment100% Examination Weight0%
Course Work Breakdown
TypeDescription% of totalAssessment Date
Reassessment Requirement
Resit arrangements are explained by the following categories;
1 = A resit is available for all components of the module
2 = No resit is available for 100% continuous assessment module
3 = No resit is available for the continuous assessment component
Indicative Reading List

  • Golemis, E.A. & Adams, P.: 2014, Protein-Protein Interactions: A Molecular Cloning Manual (2nd Edition), Cold Spring Harbor Laboratory Press, c/o Oxford University Press,
  • Lovric J.: 2011, Introducing Proteomics, Wiley-Blackwell,
  • Bonner , P.: 2006, Protein Purification, Taylor & Francis,
  • GK Walsh: 2002, Protein Biochemistry & Biotechnology, Wiley, 978471899075
  • C Hardin et al.: 2001, Cloning, Gene Expression and Protein Purification, Oxford Univ Press,
  • Walsh, G (Ed): 2009, Post-translational modification of protein biopharmaceuticals, Wiley-Blackwell,
  • Walsh, G: 2007, Pharmaceutical Biotechnology: Concepts and Applications, J Wiley & Sons UK,
Other Resources

Programme or List of Programmes
BTBSc in Biotechnology
GCBBSc in Genetics & Cell Biology
SHSAStudy Abroad (Science & Health)
SHSAOStudy Abroad (Science & Health)