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Module Specifications

Archived Version 2006 - 2007

Module Title Protein Biotechnology
Module Code BE402
School School of Biotechnology

Online Module Resources

Module Co-ordinatorDr Ciaran FaganOffice NumberX207
Level 4 Credit Rating 5
Pre-requisite None
Co-requisite None
Module Aims
Module Aims: - To review the use of enzymes in key industrial processes, both traditional and recently-evolved, with emphasis on their underlying biotechnological strengths and current limitations. - To outline how biotechnological innovations (especially protein engineering) have resulted in improved products and processes. - To provide an overview of biosensor development. - To understand the relevance of DNA recombinant technology for the production & purification of proteins and peptides. - To learn some important techniques used for the purification and analysis of peptides & proteins. - To review problems associated with large scale/scale-up production of proteins.

Learning Outcomes
Learning Outcomes: Students will gain: - An overview of the uses and advantages of enzymes in starch hydrolysis, antibiotic modification and other industrial processes - An overview of techniques and resources underpinning protein engineering - An understanding of the potential of protein engineering for the tailoring of useful proteins - An appreciation of the scope and potential of enzyme and biosensor technologies in the evolution of current, developing and innovative products/processes - An understanding of the basis of a variety of modern protein separation/analytical methodologies - An appreciation of the applications of DNA recombinant technology to the production of proteins.

Indicative Time Allowances
Lectures 30
Tutorials 2
Laboratories 0
Seminars 3
Independent Learning Time 40

Total 75
Assume that a 5 credit module load represents approximately 75 hours' work, which includes all teaching, in-course assignments, laboratory work or other specialised training and an estimated private learning time associated with the module.

Indicative Syllabus
INDUSTRIAL ENZYMOLOGY Sources and production of industrial enzymes; enzymes in detergents; starch processing and high-fructose syrup; use of enzymes for antibiotic semisynthesis; leather processing; cellulose degradation in the textile and paper industries. PROTEIN ENGINEERING Underlying technologies, incl. databases and bioinformatics; Case history - subtilisin; Insulin and its semisynthesis; Directed evolution of an enzyme. BIOSENSORS Definition & applications; biocomponents; transducers; design problems; developments in biosensors incl. examples. PROTEIN PURIFICATION & ANALYSIS Advanced Separations (Affinity, Metal-chelate (IMAC), Covalent Chromatographies Purification of Recombinant proteins (His-tag & Thioredoxin fusions , inclusion body formation etc).Genetic Manipulation of protein source for optimal protein/peptide production and stability . Capilliary Electrophoresis - Protein & Peptide Analysis. Large scale protein purification and associated problems of scale-up. Introduction to the concepts of Proteomics and Protein Microarrays BIOPHARMA & recombinant proteins Introduction to Biopharma Protein drug delivery systems
Continuous Assessment0% Examination Weight100%
Indicative Reading List
Indicative Reading List: Essential: Enzyme Technology M.F. Chaplin & C. Bucke Cambridge University Press (1990) Protein Biochemistry & Biotechnology G.K. Walsh Wiley (2002) . Cloning, Gene Expression and Protein Purification Hardin, C et al. Oxford University Press (2001). Protein Purification Techniques Roe, Simon. Oxford Press (2001) Protein Engineering Moody, P.C.E. & Wilkinson, A.J. IRL Press (1990) Supplementary: Protein Purification Methods Harris, E.L.V. & Angal, S. IRL Press (1995) Protein Purification Applications Harris, E.L.V & Angal, S. IRL Press (1994) Protein Biotechnology G.K. Walsh & D.G. Headon Wiley 1993
Programme or List of Programmes
BSSAStudy Abroad (DCU Business School)
BSSAOStudy Abroad (DCU Business School)
BTBSc in Biotechnology
ECSAStudy Abroad (Engineering & Computing)
ECSAOStudy Abroad (Engineering & Computing)
HMSAStudy Abroad (Humanities & Soc Science)
HMSAOStudy Abroad (Humanities & Soc Science)
SHSAStudy Abroad (Science & Health)
SHSAOStudy Abroad (Science & Health)