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Module Title |
Protein Biotechnology
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Module Code |
BE402
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School |
School of Biotechnology
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Online Module Resources
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| Module Co-ordinator | Dr Ciaran Fagan | Office Number | X207 |
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Level |
4
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Credit Rating |
5
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Pre-requisite |
None
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Co-requisite |
None
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Module Aims
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Module Aims:
- To review the use of enzymes in key industrial processes, both traditional and recently-evolved, with emphasis on their underlying biotechnological strengths and current limitations.
- To outline how biotechnological innovations (especially protein engineering) have resulted in improved products and processes.
- To provide an overview of biosensor development.
- To understand the relevance of DNA recombinant technology for the production & purification of proteins and peptides.
- To learn some important techniques used for the purification and analysis of peptides & proteins.
- To review problems associated with large scale/scale-up production of proteins.
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Learning Outcomes
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Learning Outcomes:
Students will gain:
- An overview of the uses and advantages of enzymes in starch hydrolysis, antibiotic modification and other industrial processes
- An overview of techniques and resources underpinning protein engineering
- An understanding of the potential of protein engineering for the tailoring of useful proteins
- An appreciation of the scope and potential of enzyme and biosensor technologies in the evolution of current, developing and innovative products/processes
- An understanding of the basis of a variety of modern protein separation/analytical methodologies
- An appreciation of the applications of DNA recombinant technology to the production of proteins.
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Indicative Time Allowances
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Hours
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Lectures |
30
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Tutorials |
2
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Laboratories |
0
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Seminars |
3
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Independent Learning Time |
40
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Total |
75
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Placements |
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Assignments |
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NOTE
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Assume that a 5 credit module load represents approximately 75 hours' work, which includes all teaching, in-course assignments, laboratory work or other specialised training and an estimated private learning time associated with the module.
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Indicative Syllabus
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INDUSTRIAL ENZYMOLOGY
Sources and production of industrial enzymes; enzymes in detergents; starch processing and high-fructose syrup; use of enzymes for antibiotic semisynthesis; leather processing; cellulose degradation in the textile and paper industries.
PROTEIN ENGINEERING
Underlying technologies, incl. databases and bioinformatics; Case history - subtilisin; Insulin and its semisynthesis; Directed evolution of an enzyme.
BIOSENSORS
Definition & applications; biocomponents; transducers; design problems; developments in biosensors incl. examples.
PROTEIN PURIFICATION & ANALYSIS
Advanced Separations (Affinity, Metal-chelate (IMAC), Covalent Chromatographies Purification of Recombinant proteins (His-tag & Thioredoxin fusions , inclusion body formation etc).Genetic Manipulation of protein source for optimal protein/peptide production and stability .
Capilliary Electrophoresis - Protein & Peptide Analysis.
Large scale protein purification and associated problems of scale-up.
Introduction to the concepts of Proteomics and Protein Microarrays
BIOPHARMA & recombinant proteins
Introduction to Biopharma
Protein drug delivery systems
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| Assessment | | Continuous Assessment | 0% | Examination Weight | 100% |
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Indicative Reading List
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Indicative Reading List:
Essential:
Enzyme Technology M.F. Chaplin & C. Bucke Cambridge University Press (1990)
Protein Biochemistry & Biotechnology G.K. Walsh Wiley (2002) .
Cloning, Gene Expression and Protein Purification Hardin, C et al. Oxford University Press (2001).
Protein Purification Techniques Roe, Simon. Oxford Press (2001)
Protein Engineering Moody, P.C.E. & Wilkinson, A.J. IRL Press (1990)
Supplementary:
Protein Purification Methods Harris, E.L.V. & Angal, S. IRL Press (1995)
Protein Purification Applications Harris, E.L.V & Angal, S. IRL Press (1994)
Protein Biotechnology G.K. Walsh & D.G. Headon Wiley 1993
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Programme or List of Programmes
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| BSSA | Study Abroad (DCU Business School) |
| BSSAO | Study Abroad (DCU Business School) |
| BT | BSc in Biotechnology |
| ECSA | Study Abroad (Engineering & Computing) |
| ECSAO | Study Abroad (Engineering & Computing) |
| HMSA | Study Abroad (Humanities & Soc Science) |
| HMSAO | Study Abroad (Humanities & Soc Science) |
| SHSA | Study Abroad (Science & Health) |
| SHSAO | Study Abroad (Science & Health) |
| Archives: | |
