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Module Specifications

Archived Version 2009 - 2010

Module Title ProteOmics and Protein Biotechnology
Module Code BE402
School School of Biotechnology

Online Module Resources

Module Co-ordinatorDr Ciaran FaganOffice NumberX207
Level 4 Credit Rating 5
Pre-requisite None
Co-requisite None
Module Aims
  • To review the use of enzymes in key industrial processes, both traditional and recently-evolved, with emphasis on their underlying biotechnological strengths and current limitations.
  • To outline how biotechnological innovations (especially protein engineering) have resulted in improved products and processes.
  • To provide an overview of biosensor development.
  • To illustrate the relevance of DNA recombinant technology for the production and purification of proteins and peptides.
  • To describe some important techniques used for the purification and analysis of peptides and proteins.
  • To review problems associated with large scale/scale-up production of proteins.


Learning Outcomes
On completion of this module, the student will be able to:
  • Discuss the uses and advantages of bulk enzymes in key industrial processes (detergents, starch processing, antibiotics and cellulose degradation)
  • Describe the basis of a variety of contemporary protein separation/analytical methodologies
  • Provide an overview of techniques and resources underpinning protein engineering
  • Illustrate, using examples, the potential of protein engineering for the tailoring of useful proteins
  • Outline the scope and potential of enzyme and biosensor technologies in the evolution of current, developing and innovative products/processes
  • Outline the applications of DNA recombinant technology to the production of proteins
  • Provide an overview of proteomics.


Indicative Time Allowances
Hours
Lectures 30
Tutorials 0
Laboratories 0
Seminars 0
Independent Learning Time 45
Total 75
Placements
Assignments
NOTE
Assume that a 5 credit module load represents approximately 75 hours' work, which includes all teaching, in-course assignments, laboratory work or other specialised training and an estimated private learning time associated with the module.

Indicative Syllabus
INDUSTRIAL ENZYMOLOGY
Sources and production of industrial enzymes; enzymes in detergents; starch processing and high-fructose syrup; use of enzymes for antibiotic semisynthesis; leather processing; cellulose degradation in the textile and paper industries.

PROTEIN ENGINEERING
Underlying technologies, incl. databases and bioinformatics; Case history - subtilisin; Insulin and its semisynthesis; Directed evolution of an enzyme.

BIOSENSORS
Definition & applications; biocomponents; transducers; design problems; developments in biosensors incl. examples.

PROTEIN PURIFICATION & ANALYSIS
Advanced Separations (Affinity, Metal-chelate (IMAC), Covalent Chromatographies Purification of Recombinant proteins (His-tag & Thioredoxin fusions , inclusion body formation etc).Genetic Manipulation of protein source for optimal protein/peptide production and stability .Capilliary Electrophoresis - Protein & Peptide Analysis.Large scale protein purification and associated problems of scale-up.Introduction to the concepts of Proteomics and Protein Microarrays

BIOPHARMA & recombinant proteinsIntroduction to Biopharma Protein drug delivery systems
Assessment
Continuous Assessment0% Examination Weight100%
Indicative Reading List
Essential:
Protein Biochemistry & Biotechnology G.K. Walsh Wiley (2002)
Cloning, Gene Expression and Protein Purification Hardin, C et al. Oxford University Press (2001).
Protein Purification Bonner, P. Taylor & Francis (2006)
Protein Purification Techniques Roe, Simon. Oxford Press (2001)
Enzyme Technology M.F. Chaplin & C. Bucke Cambridge University Press (1990)
Protein Engineering Moody, P.C.E. & Wilkinson, A.J. IRL Press (1990)

Supplementary:
Protein Purification Methods Harris, E.L.V. & Angal, S. IRL Press (1995)
Protein Purification Applications Harris, E.L.V & Angal, S. IRL Press (1994)
Resource and applications of biotechnology R. Greenshields (2003)
Microbial Proteinases H.M Kalisz in Advances in Biochemical Engineering vol 36 pp 1-65
Programme or List of Programmes
BSSAStudy Abroad (DCU Business School)
BSSAOStudy Abroad (DCU Business School)
BTBSc in Biotechnology
ECSAStudy Abroad (Engineering & Computing)
ECSAOStudy Abroad (Engineering & Computing)
GCBBSc in Genetics & Cell Biology
HMSAStudy Abroad (Humanities & Soc Science)
HMSAOStudy Abroad (Humanities & Soc Science)
SHSAStudy Abroad (Science & Health)
SHSAOStudy Abroad (Science & Health)
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