Registry

Module Specifications

Archived Version 2016 - 2017

Module Title	Proteins, Proteomics & Biopharma
Module Code	BE402
School	School of Biotechnology
Online Module Resources
Module Co-ordinator	Dr Ciaran Fagan	Office Number	X207
NFQ level	8	Credit Rating	5
Pre-requisite	None
Co-requisite	None
Compatibles	None
Incompatibles	None

Description

To describe some important techniques used for the purification and analysis of proteins. To illustrate the relevance of DNA recombinant technology to the production and purification of proteins and peptides. To review the use of enzymes in key industrial processes, noting their strengths and limitations. To show how protein engineering has led to improved products and processes. To review insulin and erythropoietin as exemplar therapeutics, and biocatalyst evolution for synthesis of a small-molecule drug. To provide an overview of biosensor development. To review recent developments in biopharmacology.

Learning Outcomes

1. Discuss the applications of bulk enzymes in key industrial processes (e.g. detergents, starch processing).
2. Describe examples of the improvement of key proteins by protein engineering strategies and the potential for process innovation using novel biocatalysts.
3. Outline the scope and potential of biosensor technologies.
4. Outline the applications of DNA recombinant technology to the production and purification of proteins.
5. Provide an overview of proteomics and proteomic analysis.
6. To describe developments in biopharmacology.

*Type*	*Hours*	*Description*
Workload	Full-time hours per semester
Lecture	24	No Description
Independent Study	101	No Description
Total Workload: 125

All module information is indicative and subject to change. For further information,students are advised to refer to the University's Marks and Standards and Programme Specific Regulations at: http://www.dcu.ie/registry/examinations/index.shtml

Indicative Content and Learning Activities

INDUSTRIAL ENZYMES AND THEIR USES
Sources and production of industrial enzymes; enzymes in detergents; starch processing and high-fructose syrup; use of enzymes for antibiotic semisynthesis and related purposes; enzyme uses in cellulose treatment and other situations.

PROTEIN ENGINEERING
Case histories – subtilisin and other enzymes used in industrial processes.

BIOSENSORS
Definition & applications; design problems; developments in biosensors incl. examples.

PROTEIN PURIFICATION & ANALYSIS
Advanced Separations; Purification of Recombinant proteins (His-tag & Thioredoxin fusions, inclusion body formation etc); Genetic Manipulation of protein source for optimal protein/peptide production and stability; Capillary Electrophoresis - Protein & Peptide Analysis; Introduction to Proteomics.

BIOPHARMA & RECOMBINANT PROTEINS
Introduction to Biopharmacology and the importance of protein glycosylation; Erythropoietin (EPO); Insulin production and improvement via protein engineering; Biocatalyst evolution for pharmaceutical synthesis. Overview of some important biotherapeutics.

Assessment Breakdown
Continuous Assessment	0%	Examination Weight	100%

Type	Description	% of total	Assessment Date
Course Work Breakdown

Reassessment Requirement
Resit arrangements are explained by the following categories; 1 = A resit is available for all components of the module 2 = No resit is available for 100% continuous assessment module 3 = No resit is available for the continuous assessment component
Unavailable

Indicative Reading List

Golemis, E.A. & Adams, P.: 2014, Protein-Protein Interactions: A Molecular Cloning Manual (2nd Edition), Cold Spring Harbor Laboratory Press, c/o Oxford University Press,
Lovric J.: 2011, Introducing Proteomics, Wiley-Blackwell,
Bonner , P.: 2006, Protein Purification, Taylor & Francis,
GK Walsh: 2002, Protein Biochemistry & Biotechnology, Wiley, 978471899075
C Hardin et al.: 2001, Cloning, Gene Expression and Protein Purification, Oxford Univ Press,
Walsh, G (Ed): 2009, Post-translational modification of protein biopharmaceuticals, Wiley-Blackwell,
Walsh, G: 2007, Pharmaceutical Biotechnology: Concepts and Applications, J Wiley & Sons UK,

Other Resources

None

Programme or List of Programmes

BT	BSc in Biotechnology
GCB	BSc in Genetics & Cell Biology
SHSA	Study Abroad (Science & Health)
SHSAO	Study Abroad (Science & Health)

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